山西猪伪狂犬病毒gE基因主要抗原区的遗传变异分析  

Genetic Variation Analysis of gE Antigen Epitope of Pseudorabies Virus Isolated from Shanxi Area

作　　者：孟帆[1] 樊振华[1] 吴忻[1] 姚敬明[1] 王娟萍[1] 米瑞娟[1] 薛翼鹏[1] 赵岳[1] 

机构地区：[1]山西省农业科学院畜牧兽医研究所,山西太原030032

出　　处：《山西农业科学》2017年第11期1844-1848,共5页Journal of Shanxi Agricultural Sciences

基　　金：山西省重点研发计划项目(201603D221023-1);山西省农业科学院畜牧兽医研究所所级项目(XMS201503)

摘　　要：根据Gen Bank中收录的PRV Becker株的gE基因序列,设计了合成1对引物,对山西PRV流行株包含主要抗原表位区的gE部分基因进行了PCR扩增,并进行克隆与序列分析。经测序,山西PRV流行株包含主要抗原表位区的gE部分基因核苷酸序列长度为997 bp,编码322个氨基酸。测序后对山西流行株包含主要抗原表位区的gE基因核苷酸序列和推导出的氨基酸序列与18株PRV参考株进行了遗传变异分析,结果显示,山西PRV流行株包含主要抗原表位区的gE基因核苷酸序列与18株PRV参考株之间同源性为97.8%~100%,其中与YY株(湖南)、SC-1-2015株(四川)、HNXX2012株(湖北)、HN-DZ株(广东)及DL14-08株(长春)同源性最高,均为100%,与75V19株(比利时)同源性最低,为97.8%。山西PRV流行株包含主要抗原表位区的gE基因核苷酸序列推导的氨基酸序列与18株PRV参考株之间同源性为96.1%~100%,其中与YY株(湖南)、SC-1-2015株(四川)、HNXX2012株(湖北)、HN-DZ株(广东)及DL14-08株(长春)同源性最高,均为100%,与Rice(美国)同源性最低,为96.1%。山西PRV流行株包含主要抗原表位区的gE基因序列的遗传进化分析表明,山西PRV流行株属于2012年以来分离的PRV变异株群,与YY株和HNXX2012株亲缘关系较近,与欧洲和美洲分离株亲缘关系较远。Using a pair of primers designed according to the gE nucleotide sequence of PRV Becker strain from Gen Bank,the part of gE gene of Shanxi PRV strain containing the main antigenic regions encoding were amplified by PCR,and then PCR product was cloned and sequenced,a fragment of 997 bp carried out could encode 322 amino acids.The part of gE gene nucleotide sequence of Shanxi PRV strain containing the main antigenic regions encoding and amino acid sequence deduced were used to analysis the genetic variation with 18 PRV strains.The results showed that the homologies of nucleotide sequences of Shanxi PRV strain to the 18 PRV strain were 97.8%-100%,the highest homology was 100% between Shanxi PRV strains and YY strains（Hunan）,SC-1-2015 strain（Sichuan）,HNXX2012 strain（Hubei）,HN-DZ strain（Guangdong）and DL14-08 strain（Changchun）,the lowest homology was 97.8% between Shanxi PRV strain and 75 V19 strain（Belgium）.The homologies of amino acid sequences of Shanxi PRV strains to the 18 PRV strain were 96.1%-100%,the highest homology was 100% between Shanxi PRV strain and YY strains（Hunan）,SC-1-2015 strain（Sichuan）,HNXX2012 strain（Hubei）,HN-DZ strain（Guangdong）and DL14-08 strain（Changchun）,the lowest homology was 96.1% between Shanxi PRV strain and Rice（America）.The genetic evolution analysis showed that Shanxi PRV strain belonged to the PRV mutation group isolated after 2012,it had the closer relationship with YY strains and HNXX2012 strains,and the farther relationship with the European strains and the American strains.

关 键 词：猪伪狂犬病毒 GE基因 抗原区 遗传变异 

分 类 号：S858.28[农业科学—临床兽医学]