microRNA-192在晚期糖基化终产物诱导人腹膜间皮细胞上皮-间叶转化中的作用  

The role of microRNA-192 in progression of advanced glycation end products-induced epithelial-to-mesenchymal transition of human peritoneal mesothelial cells

作　　者：邵维斌 路萍 夏春英 李忻 荀康 冯松涛 赵倩 SHAO Weibin;LU Ping;XIA Chunying;LI Xin;XUN Kang;FENG Songtao;ZHAO Qian(Department of Nephrology , Zhenjiang First People＇s Hospital , Zhenjiang , Jiangsu , 212002)

机构地区：[1]江苏省镇江市第一人民医院肾脏内科,江苏镇江212002

出　　处：《实用临床医药杂志》2017年第19期95-98,共4页Journal of Clinical Medicine in Practice

基　　金：江苏省镇江市科技项目(SH2013051)

摘　　要：目的探讨microRNA-192(miR-192)对晚期糖基化终产物(AGEs)诱导人腹膜间皮细胞上皮-间叶转化(EMT)的调控作用。方法人腹膜间皮细胞、转染miR-192抑制物后人腹膜间皮细胞和转染miR-192抑制物阴性对照的人腹膜间皮细胞在含AGEs的培养基培养72 h,以M199培养基和含80 m M BSA的M199培养基为对照,然后运用实时荧光定量PCR法检测miR-192和mRNA的表达情况,运用蛋白质印迹法检测蛋白的表达情况。结果在AGEs刺激后,人腹膜间皮细胞miR-192、胶原蛋白I(Collagen I)mRNA、α-平滑肌肌动蛋白(α-SMA)mRNA和蛋白表达水平显著上升(P<0.05),而E-钙黏蛋白(E-cadherin)mRNA和蛋白表达水平显著下降(P<0.05)。与转染miR-192抑制物阴性对照的人腹膜间皮细胞相比,转染miR-192抑制物的人腹膜间皮细胞miR-192、Collagen I mRNA、α-SMAmRNA和蛋白表达水平显著下降(P<0.05),而E-cadherin mRNA和蛋白表达水平显著上升(P<0.05)。结论 AGEs可能通过上调miR-192表达诱导人腹膜间皮细胞EMT。miR-192抑物可能通过下调miR-192表达阻止AGEs诱导人腹膜间皮细胞EMT。miR-192在AGEs诱导人腹膜间皮细胞EMT中起重要调控作用。ObjectiveTo investigate the role of microRNA 192 （miR 192） in the regulation of epithelial mesenchymal transition induced by advanced glycation end products （AGEs） in human peritoneal mesothelial cells. MethodsThe human peritoneal mesothelial cells, the human peritoneal mesothelial cells transfected by miR 192 inhibitor and the human peritoneal mesothelial cells transfected by miR 192 inhibitor negative control were cultured for 72 hours in culture medium containing AGEs （80mM）; M199 medium and medium with M199 medium containing 80mM BSA as negative control, the expression of miR 192 and mRNA was detected by real time quantitative PCR, and the expression of miR 192 and mRNA was detected by Western blotting. ResultsAGEs significantly upregulated the expression of miR 192, Collagen I mRNA, α smooth muscle actin（α SMA） mRNA and protein（P〈0.05）, while significantly downregulated the E cadherin mRNA and protein expression（P〈0.05）. Compared with human peritoneal mesothelial cells transfected by miR 192 inhibitor, the expression of miR 192 and Collagen I mRNA and α SMA mRNA and protein was decreased （P〈0.05）, while E cadherin mRNA and protein expression was significantly increased （P〈0.05）. ConclusionAGEs may induce EMT of human peritoneal mesothelial cells by upregulation of miR 192 expression. MiR 192 inhibitor may prevent AGEs inducing EMT of human peritoneal mesothelial cells by down regulation of miR 192 expression, and may play an important regulatory role in EMT of human peritoneal mesothelial cells induced by AGEs.

关 键 词：晚期糖基化终产物 间皮细胞 上皮-间叶转化 microRNA-192 

分 类 号：R459.5[医药卫生—治疗学]