无偿献血者HCV核心区氨基酸置换情况分析  

Patterns of amino acid substitution in hepatitis C virus core region among Chinese volunteer blood donors

作　　者：林红[1] 邵雷[1] 胡文佳[1] 王金花[1] 朱珊珊 陈慧[1] 朱绍汶 

机构地区：[1]江苏省血液中心,江苏南京210042

出　　处：《中国输血杂志》2017年第9期1004-1006,共3页Chinese Journal of Blood Transfusion

基　　金：江苏省"六大人才高峰"项目(2014-WSN-002);江苏省第五期"333高层次人才培养工程"资助项目(LGY2016045)

摘　　要：目的研究感染HCV的无症状献血者基因型和1b基因型的核心区氨基酸置换情况。方法用巢式PCR方法扩增91个HCV RNA阳性献血者标本,根据直接测序得到的序列用Mega6.0软件进行序列比对和进化树构建,分析基因型和氨基酸置换情况。结果在78个测序结果清晰的样品中,66个为基因型1b(84.6%),其他基因型为1a(1,1.3%)、2a(7,8.9%)、3b(2,2.6%)和6a(2,2.6%)。62个1b样品的核心区蛋白(aa68-120)发生17处氨基酸置换。第70位R70Q、R70P和R70H各1例;第91位L91M置换非常普遍,为87.9%。结论本研究中献血者感染的HCV基因型主要为1型,尤其是1b为优势基因型。首次发现中国献血者感染的HCV核心区蛋白的氨基酸置换具有独特性。Objective To estimate the prevalence of genotypes and amino acid substitutions in the HCV core region genotype 1 b in blood donors who always were asymptomatic with HCV infection.Methods A total of 91 HCV RNA positive samples were amplified by nest-PCR.Then,they were genotyped and the mutations were analyzed by phylogenetic tree according to sequences.Results In 78 samples with clear sequences,66 samples were classified into HCV subtype 1 b（84.6%） followed by subtype 2 a（10.3%）,6 a（3.85%）,1 a（1,1.3%）,2 a（7,8.9%）,3 b（2,2.6%） and 6 a（2,2.6%）.Seventeen amino acid substitutions from aa68-120 of Core region were detected in 62 samples.R71 Q,R70 P and R70 H were1（1.6%） respectively,but L90 M（87.9%） was common.Conclusion HCV genotype 1,especially 1 b,was the dominant genotype in this investigation.The patterns of amino acid substitution in the core region in blood donors were distinctive.

关 键 词：HCV基因型 HCV核心区 氨基酸置换 献血者 

分 类 号：R457.11[医药卫生—治疗学] R446.6[医药卫生—临床医学]