一例类同种抗-D抗体鉴定及输血效果评估  

A case of autoantibody with mimicking anti-D specificity and to evaluate its efficacy of blood transfusion

作　　者：于建 向东[2] 刘曦[2] 李志强 

机构地区：[1]上海市交通大学附属第六人民医院输血科,上海200233 [2]上海市血液中心

出　　处：《中国输血杂志》2017年第9期1067-1069,共3页Chinese Journal of Blood Transfusion

摘　　要：目的通过确定患儿血清中存在的不规则抗体的性质及效价,配合型输血,以评估其输血疗效。方法应用试管法检测ABO及RhD血型,使用微柱凝胶法、凝聚胺、抗球蛋白法对患儿血清及放散液中的不规则抗体进行筛查鉴定,并测定血清中抗体的效价。结果患儿血型为"O"CcDEe,血清及放散液中存在类同种抗-D,其血清中抗体效价为8。随着给予患儿输注RhD阴性红细胞输注剂量与次数增加,患儿血红蛋白值逐渐增高,类同种抗-D效价逐渐减低。结论应根据类同种自身抗体特异性,选择输注其对应抗原阴性红细胞,才能确保临床输血安全性与有效性。Objective Evaluate the efficacy of transfusion by determining the nature,potency of irregular antibodies and matched transfusion in pediatric patients＇ serum.Methods The ABO and RhD blood groups were detected by the tube method,and the irregular antibodies in the serum and the effluent of pediatric patients were screened and identified by microcolumn gel method,polybrene test and the method of anti globulin.Results The pediatric patients＇ ABO and RhD blood groups are＂O＂and＂CcDEe＂respectively.Specific autoantibodies D were found in the serum and elution.The antibodie titer in serum was 8.With the increasing transfusion dose and transfusion times of RhD negative red blood cell for the pediatric patients,their hemoglobin were improved and the titers of antibodies decreased accordingly.Conclusion IIn order to ensure the safety and effectiveness of clinical transfusion,we should select the corresponding antigen negative red blood cells according to the specificity of the same kind of autoantibodies.

关 键 词：RHD血型 同种抗体 自身抗体 输血疗效 

分 类 号：R457.11[医药卫生—治疗学]