口服牛Ⅱ型胶原蛋白诱发类风湿关节炎大鼠模型的建立及评价  

The Establishment and Accessing of Rheumatoid Arthritis Model in Rat by Oral Bovine Collagen Type Ⅱ

作　　者：关丽[1] 毋丹丹 胡东生[2] 刘冬舟 刘志刚[1,2] 

机构地区：[1]深圳市罗湖区人民医院风湿免疫科,广东深圳518001 [2]深圳大学过敏反应与免疫学研究所,广东深圳518060 [3]深圳市人民医院风湿免疫科,广东深圳518020

出　　处：《江西师范大学学报（自然科学版）》2017年第5期513-516,共4页Journal of Jiangxi Normal University(Natural Science Edition)

基　　金：国家自然科学基金(81271950);深圳市科技计划基础研究(JCYJ20130402151227168)资助项目

摘　　要：按照动物建模方案分别给不同组大鼠灌胃一定剂量的阿司匹林,再给大鼠灌胃牛II型胶原蛋白诱导其产生类风湿关节炎;建模期间进行踝关节直径的测量及关节炎指数评分;然后ELISA实验检测大鼠血清中特异性抗体(IgE、IgG)和炎症因子(IL-6、TNF-α和IFN-γ);再进一步观察大鼠踝关节组织病理改变.结果表明:经过不同剂量阿司匹林(5、10 mg·kg-1)处理的大鼠,用牛II型胶原蛋白灌胃后,大鼠踝关节呈现不同程度的肿胀及关节炎症状;10 mg·kg-1处理的大鼠炎症更明显,其血清中特异性抗体(IgE、IgG)和炎性细胞因子IL-6、TNF-a也显著增加,而细胞因子IFN-r显著降低,同时踝关节组织也发生明显炎症病变.这表明10 mg·kg-1处理的大鼠要比5 mg·kg-1处理大鼠更易用于口服食物抗原牛II型胶原蛋白类风湿关节炎的建成.An RA animal model was developed by gavage feeding with bovine collagen type II and aspirin,and tail vein injection with lipopolysaccharide. The diameter of ankle were measured and the score of the arthritis. The concentration of IgE,IgG,IL-6,TNF-α and IFN-γ in serum by ELISA test. The inflammation in the ankle joints was evaluated by light microscopy and the ankle histopathological changes. The results show that Rats treated with high doses of aspirin（ 10 mg·kg^（-1）） can be successfully induced by oral bovine collagen type II. The ankle of rat presents obvious swelling and arthritis symptoms. High levels of proinflammatory cytokines and specific antibodies were detected in the sera. The histology showed an inflammatory feature in the ankle joints. Rats treated with high doses of aspirin（ 10 mg·kg^（-1）） can be successfully induced by oral bovine collagen type II.

关 键 词：类风湿关节炎 食物抗原 口服 II型胶原蛋白 动物模型 

分 类 号：R593.22[医药卫生—内科学]