复方茵陈合剂总黄酮测定方法研究  

Research on the determination of total flavonoids in compound Yinchen mixture

作　　者：刘晓玲[1] 蔡小辉[1] 倪晓霞[1] 王庆芬[1] LIU Xiaoling;CAI Xiaohui;NI Xiaoxia;WANG Qingfen(No . 175 Hospital of PLA , Zhangzhou 363000 , China)

机构地区：[1]解放军175医院/厦门大学附属东南医院,福建漳州363000

出　　处：《药学实践杂志》2017年第6期547-550,共4页Journal of Pharmaceutical Practice

基　　金：福建中医药大学临床专项校管课题(XB2016067)

摘　　要：目的建立紫外-可见分光光度法测定复方茵陈合剂中总黄酮含量。方法以芦丁作为对照,依次用直接测定法、氯化铝-醋酸钠显色法、硝酸铝显色法,探究最优测定方法。结果本品最优显色法为硝酸铝显色法,测定波长为508 nm,在0.012 5~0.062 6 g/L范围内线性关系良好,线性回归方程为Y=12.127 30 X+0.000 14(r=0.999 9),加样回收率平均值为99.49%(n=9,RSD为0.84%)。结论硝酸铝显色法可作为复方茵陈合剂中总黄酮的含量测定方法,该法简便、快速、稳定可靠。Objective To establish a method for the determination of the total flavonoids content in compound Yinchen mixture by UV spectrophotometry.Methods Using rutin as comparison, three coloration methods were studied to find the optimal assay method.Results The sample was detected at 508 nm wavelength by NaNO2-Al（NO3）3-NaOH reaction with rutin as reference. The rutin content had a liner relationship in the range of 0.012 5-0.062 6 g/L （n=9,r=0.999 9）, and the average recovery rate was 99.49% with RSD of 0.84%. Conclusion The NaNO2-Al（NO3）3-NaOH coloration method is proved to be simple, quick, stable and reliable for the determination of total flavonoids in compound Yinchen mixture.

关 键 词：紫外分光光度法 复方茵陈合剂 总黄酮 含量测定 

分 类 号：R284.1[医药卫生—中药学]