尖叶石竹种子的EMS和^(60)Co-γ诱变  

Analysis of EMS and ^(60)Co-γmutagenesis of Dianthus spiculifolius seeds

作　　者：龚束芳 刘恩慧 姜童童 周爱民 王金刚 Gong Shu-fang;Liu En-hui;Jiang Tong-tong;Zhou Ai-min;Wang Jin-gang(College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, Heilongjiang,China.)

机构地区：[1]东北农业大学园艺园林学院,黑龙江哈尔滨150030

出　　处：《草业科学》2017年第11期2226-2234,共9页Pratacultural Science

基　　金：国家重点研发计划项目(2016YFC0500300);哈尔滨市应用技术研究与开发项目(2015RQQXJ021)

摘　　要：为了获得更多尖叶石竹(Dianthus spiculifolius)新品种,本研究利用EMS和^(60)Co-γ射线对尖叶石竹种子进行了诱变处理。结果显示,处理时间为16h时,1.0%的EMS浓度接近诱变的半致死剂量,变异率达到7.0%。当剂量率为0.1Gy·min^(-1)时,300Gy的^(60)Co-γ射线接近诱变的半致死剂量,变异率达到4.2%。通过表型观察共获得了1 038株M1代变异植株,其中,EMS诱变处理获得了95株,^(60)Co-γ射线诱变处理获得了943株。本研究为丰富尖叶石竹种质资源提供了帮助,而且为石竹属其他植物的诱变研究提供了技术参考。In order to develop new varieties of Dianthus spiculifolius, we mutagenized seeds using EMS and 60Co γ rays. Treatment of seeds for 16 h with 1% EMS, close to the half lethal dose, resulted in a mutation rate of 7.0%. Seeds treated with 300 Gy 60Co γ rays （dose rate 0.1 Gy·min-1, close to the half lethal dose, resulted in a mutation rate of 4.2%. We obtained 1 038 phenotypic mutants in the M1 generation, of which 95 were derived from seeds treated with EMS, and 943 were obtained following irradiation of the seeds. This study has enriched the germplasm resources of D. spiculifolius and has provided a technical reference for the mutagenesis of other Dianthus species.

关 键 词：种子 诱变 变异率 甲基磺酸乙酯 ^60 CO-Γ射线 突变体 石竹属 

分 类 号：S682.19[农业科学—观赏园艺]