北细辛悬浮培养体系的建立及优化  

Establishment and optimization of a suspension culture system for culturing Asarum heterotropoides

作　　者：刘思妤[1] 杨悦[1] 王鹰 王丽娟[1] 吴秀菊[1] Liu Si-yu;Yang Yue;Wang Ying;Wang Li- juan;Wu Xiu-ju(College of Life Sciences,Northeast Agricultural University, Harbin 150030, Heilongjiang,China.)

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《草业科学》2017年第11期2254-2260,共7页Pratacultural Science

基　　金：国家基础科学人才培养基金能力培养与科研训练项目(J1210069);黑龙江省教育厅科学技术研究项目计划(12531030);哈尔滨市科技局科技创新人才研究专项(2012RFLXN003)

摘　　要：以北细辛(Asarum heterotropoides)幼嫩叶柄诱导的愈伤组织为材料,初步建立悬浮培养体系,同时探讨接种量、光照、培养液量、转速、蔗糖浓度、激素配比等因素对细胞生物量的影响。结果表明,100mL的1/2MS液体培养基中,2g愈伤组织是最适接种量,蔗糖浓度为30g·L^(-1),摇床转速150r·min^(-1),0.6mg·L^(-1) 6-BA+0.3mg·L^(-1) NAA,全天光照培养时细胞生长量最大。北细辛悬浮细胞生长曲线大致为"S"型,悬浮细胞的最佳收获周期为15d。经悬浮培养后可快速获得细胞团及多种形态的单细胞,可为北细辛悬浮细胞的大规模生产提供依据,并进一步提升北细辛的应用价值。Using the callus induced by young petioles of Asarum heterotropoides, a suspension culture system was established. Then the effects of the amount of inoculum, lighting time, amount of culture fluid, speed of rotation, and concentrations of sucrose and plant growth regulators on the cell suspension culture of A. heterotropoides were studied. The results showed that the optimal conditions for callus suspension culture were 2 g calli inoculated in 100 mL 1/2 MS liquid medium containing 0.6 mg·L-1 6 BA and 0.3 mg·L-1 NAA, a rotation speed of 150 r·min-1, 30 g·L-1 sucrose, and exposure to whole day light. The growth curve of suspension cells of A. heterotropoides was S type. The best harvest time of suspension cells was 15 d. Through the suspension culture, the cell masses and various forms of single cells could be obtained rapidly. These findings provide the basis for large scale culture of A. heterotropoides suspension cells so as to enhance the application value of A. heterotropoides.

关 键 词：北细辛 愈伤组织 悬浮培养 细胞生物量 培养条件 大规模生产 

分 类 号：S567.239[农业科学—中草药栽培]