双黄连预处理对LPS致小鼠内毒素血症模型SOD和MDA水平的影响  

The Effect of Shuanghuanglian Pre-treatment on the Level of Endoxemia SOD and MDA Induced by LPS in Mice

作　　者：张辉 王俊[3] 甘平 罗厚强[1,5] ZHANG Hui;WANG Jun;GAN Ping;LUO Hou-qiang(l.College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 43007;College of Animal Science, Anhui Science and Technology University, Fengyang, Anhui 233100;Jingshan Animal Disease Control Center, Jingmen, Hubei 431800;Jiangxi Animal Disease Prevention and Control Center, Nanchang, Jiangxi 330096;Animal Science Department, Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang 325006)

机构地区：[1]华中钇业大学动物医学院,湖北武汉430070 [2]安徽科技学院动科院,安徽凤阳233100 [3]京山县动物疫病预防控制中心,湖北荆门431800 [4]江西省动物疫病预防控制中心,江西南昌330096 [5]温州科技职业学院动科系,浙江温州325006

出　　处：《温州科技职业学院学报》2016年第3期21-24,共4页Journal of WenZhou Vocational College of Science And Technology

基　　金：温州市2014年公益性科技计划项目（N20140041）;2016年温州科技职业学院博士科研启动项目（201604）

摘　　要：目的：观察双黄连口服液预处理对LPS致小鼠内毒素血症模型SOD和MDA水平的影响.方法： 将25只昆明小鼠随机分成空白对照组、试验对照组、试验组（Ⅰ 、Ⅱ 、Ⅲ ）.试验Ⅰ 、Ⅱ 、Ⅲ组分别口服 双黄连0.1ml/100ml、0.4ml/100ml、1.6ml/100ml.在5周后处理小鼠,心脏采血,制成血涂片备用.结 果：试验组与空白对照组比较S0D明显降低, MDA明显升高;试验Ⅰ组与试验对照组比较SOD活性显著升高, MDA含量显著降低;试验Ⅱ组与试验Ⅰ组比较SOD活性显著升高,MDA含量显著降低;试验Ⅲ组与试验Ⅱ组 比较SOD活性显著降低,MDA含量显著升高.结论：双黄连具有抑制内毒素血症抗氧化降低的作用,且在适 量范围内双黄连的抑制作用随其含量的增加而增加,当双黄连的含量超过一定值后,其抑制作用反而随其 含量的增加而降低.To investigate the effect of Shuanghuanglian pre-treatment on the level of endoxemia SOD and MDA induced by LPS in mice. A total of twenty five mice were randomly allocated into several groups, blank comparison group, experimental comparison group and experimental groups （ Ⅰ 、Ⅱ and Ⅲ）- The experimented groups Ⅰ 、Ⅱ and Ⅲ were injected with the Shuanghuanglian with concentration of 0.0ml/100ml, 0.4ml/100ml, 1.6ml/100ml, respectively. After 5 weeks, animals were slaughtered to collect blood samples from the heart and to separate the blood plasma to evaluate antioxidant capability for further analysis. Results revealed that the SOD activities between the blank comparison group and the experimental group decrease significantly, while the level of MDA increased significantly. When the differences between test control group and group I are compared,the SOD activity increased significantly, while the level of MDA decreased significantly. The difference between group II and group I increased significantly in SOD activity, while the level of MDA decreased significantly. When compared in the group Ⅱ and and Ⅲ the SOD activity decreased significantly, while the level of MDA increased significantly. In conclusion, Shuanghuanglian can restrain the effect of endotoxin that can reduce the antioxidant effect of endoxemia in mice. This inhibiting effect will increase with the increased dose of Shuanghuanglian in moderate range. While the content of Shuanghuanglian exceeds a certain value, the inhibiting effect will decrease with the increased dose of Shuanghuanglian.

关 键 词：双黄连口服液 内毒素 血浆 SOD MDA 

分 类 号：R285[医药卫生—中药学]