几种超分辨率荧光显微技术  

Introduction of several super-resolution microscopy

作　　者：王旭[1] 李春香[1] 孟祥宁[1] 

机构地区：[1]哈尔滨医科大学医学遗传学研究室,150081

出　　处：《国际遗传学杂志》2017年第5期302-306,共5页International Journal of Genetics

基　　金：国家国际科技合作专项（2013DFA31610）;教育部“创新团队发展计划”（IRT1230）;国家自然科学基金（81402476、81572915、81372784）

摘　　要：传统的光学显微技术受限于瑞利/阿贝准则无法分辨出200 nm以下的结构,而超分辨率荧光显微技术能突破衍射极限对亚细胞结构进行观测.这些超分辨率荧光显微技术大体上可以分为3类：①基于结构光照明的超分辨率成像技术,如受激发射损耗显微技术和饱和结构照明显微术;②基于单分子定位的超分辨率成像技术,如随机光学重构显微术和光激活定位显微术;③基于漂白、闪烁的超分辨率成像技术.本文了上述3类超分辨率荧光显微技术提高成像分辨率的方法及应用,并对其发展趋势进行展望.Limited by the Abbe/Rayleigh criteria of light,traditional light microscopy can＆#39;t differentiate structures less than 200nm in diameter.Super-resolution fluorescence microscopy can observe subcellular structure by breaking diffraction limit.These super-resolution techniques can be generally divided into three primary classes：①patterned illumination-based super-resolution imaging,such as stimulated emission depletion and saturated structured illumination microscopy;②single-molecule localizationbased super-resolution imaging,such as stochastic optical reconstruction microscopy and photo-activation localization microscopy;③bleaching/blinking-based super-resolution imaging.This review summarizes the methods and applications of these three kinds of super-resolution fluorescence microscopy and discusses the perspective of this new technology.

关 键 词：超分辨率荧光显微镜 受激发射损耗显微术 饱和结构照明显微术 随机光学重构显微术 光激活定位显微术 基于漂白 闪烁的超分辨率成像技术 

分 类 号：R312[医药卫生—基础医学]