中度嗜盐菌群对酸性大红GR的脱色性能研究  

Decoloring acid scarlet GR by a moderately halophilic bacterial consortium

作　　者：郭光 田芳 刘妍 刘廷凤 丁克强 刘翀[2] 

机构地区：[1]南京工程学院环境工程学院,江苏南京211167 [2]中国农业科学院农业环境与可持续发展研究所,北京100081

出　　处：《微生物学通报》2017年第11期2567-2574,共8页Microbiology China

基　　金：国家自然科学基金项目(No.31600091;51608257;31402117);南京工程学院人才引进项目(No.YKJ201528);南京工程学院大学生科技创新项目(No.TB201712001)~~

摘　　要：【目的】获得能够在高盐环境下脱色偶氮染料的高效脱色菌群,应用于印染废水的生物处理。【方法】采用在5%盐度培养基富集的方法,从印染废水的活性污泥中富集能够在5%盐度下脱色酸性大红GR的嗜盐混合菌群,利用高通量测序方法研究其群落结构,利用静置培养的方法研究其脱色性能。【结果】该菌群可以在5%盐度、静置培养下15 h内将100 mg/L的酸性大红GR几乎完全脱色,主要由Halomonas、Salinicoccus、Nitratireductor和Aequorivita等4个属组成,Halomonas是主要的脱色菌。高浓度的Na NO_3、Na_2SO_4和Na Cl抑制菌群的脱色,其中Na NO_3抑制作用最强。该菌群的最佳脱色条件是在p H 7.0、盐度5%、30°C脱色效果最好,可脱色直接耐黑G和分散深蓝S-3BG等偶氮染料,并且具有连续脱色的能力。【结论】嗜盐菌群在处理偶氮染料废水中具有良好的应用价值。[Objective]To obtain efficient decoloration bacterial consortium,which were able to degrade azo dyes in high salt environment and to biodegrade the dyeing wastewater.[Methods]A moderately halophilic bacterial consortium degrading of acid scarlet GR was enriched from activated sludge of dyeing wastewater.The community structure of the bacterial consortium was studied by high-throughput sequencing method,and the decoloring performance was studied under static condition.[Results]The moderately bacterial consortium was mainly composed by Halomonas,Salinicoccus,Nitratireductor and Aequorivita,Halomonas was the main decoloring bacteria.The degradation was inhibited by high concentration of Na Cl,Na_2SO_4 and Na NO_3,of which the strongest inhibition is Na NO_3.The optimal decolorization conditions were as follows：p H 7.0,30℃,5%Na Cl.The moderately halophilic bacterial consortium could also degrade direct black 19 and disperse deep blue S-3BG.After continuous bleaching for 5 rounds,the degradation rate still reached over 98%.[Conclusion]The moderately halophilic bacteria consortium has strong potential to treat azo dye contaminated wastewater.

关 键 词：酸性大红GR 脱色 中度嗜盐菌群 盐度 偶氮染料 

分 类 号：X791[环境科学与工程—环境工程]