羊肚菌白霉病发生对土壤真菌群落结构的影响  

Effects of Morchella white mold disease on soil fungal community structure

作　　者：陈诚[1] 李强[2] 黄文丽[2] 王剑[1] 伏荣桃[1] 罗曦[1] 卢代华[1] 

机构地区：[1]四川省农业科学院植物保护研究所,四川成都610066 [2]四川省农业科学院生物技术核技术研究所,四川成都610066

出　　处：《微生物学通报》2017年第11期2652-2659,共8页Microbiology China

基　　金：四川省"十三五"农作物及畜禽育种攻关项目(No.2016NYZ0053-1);四川省财政创新能力提升工程项目(No.2016GYSH-014);四川省科技支撑计划项目(No.2016KZ0006)~~

摘　　要：【目的】了解羊肚菌白霉病发生对土壤真菌群落结构的影响。【方法】采用Illumina Mi Seq高通量测序技术,对未栽培羊肚菌土壤、正常生长羊肚菌和染白霉病羊肚菌根际土真菌群落结构进行研究。【结果】9个样品共得到393 347条有效序列。未栽培羊肚菌土壤真菌多样性指数和丰度显著大于羊肚菌根际土,染病羊肚菌根际真菌丰度和多样性大于正常生长的羊肚菌,各样品的群落组成和优势类群有较大差异。【结论】羊肚菌栽培后真菌多样性降低,白霉病发生后根际真菌种类增多,土壤真菌群落结构发生变化,优势真菌类群也产生了较大变化。[Objective] To understand the effect of the Morchella white mold on soil fungal community structure. [Methods] Illumina Mi Seq high throughput sequencing technology was used to analyze the fungal community structure of control soil, rhizosphere soil of diseased and healthy Morchella. [Results] The results showed that a total of 393 347 valid sequences were obtained from 9 samples collected. The fungal abundance and diversity of control soil were the highest among all the samples, followed by that of diseased Morchella, which were significantly higher than that of healthy Morchella. The community composition and dominant taxa of different samples varied significantly. [Conclusion] The diversity of soil fungi decreased in the field of cultivated Morchella. While, the occurrence of white mold disease lead the abundance of rhizosphere fungi increased, the community structure changed, and the dominant fungi groups varied.

关 键 词：羊肚菌 白霉病 MiSeq高通量测序 真菌群落结构 

分 类 号：S154.3[农业科学—土壤学] S436.46[农业科学—农业基础科学]