蒲公英乙酸乙酯部位化学成分研究  

Chemical constituents of ethyl acetate extract from dandelion

作　　者：马晓玲[1] 张雪琼 李心愿 吴珊珊 

机构地区：[1]湖北第二师范学院植物抗癌活性物质提纯与应用湖北省重点实验室,湖北武汉430205 [2]武汉理工大学化学化工与生命科学学院,湖北武汉430070

出　　处：《中国医院药学杂志》2017年第21期2139-2141,共3页Chinese Journal of Hospital Pharmacy

基　　金：重点实验室开放课题基金项目(编号:HLPAI2014005)

摘　　要：目的:研究蒲公英乙酸乙酯部位的化学成分。方法:采用渗漉法提取蒲公英有效成分,硅胶柱层析、反相高效液相色谱法及半制备高效液相色谱法对提取物进行分离和纯化,依据单体化合物的理化性质及核磁共振谱图确定其化学结构。结果:从蒲公英乙酸乙酯部位分离得到6个化合物,分别是棕榈酸(Ⅰ)、咖啡酸(Ⅱ)、咖啡酸乙酯(Ⅲ)、对羟基苯乙酸乙酯(Ⅳ)、阿魏酸(Ⅴ)和blumenol A(Ⅵ)。结论:化合物Ⅵ为首次从蒲公英中分离得到。OBJECTIVE To study the chemical constituents of ethyl acetate extract from dandelion.METHODS Percolation method was used to extract effective components of dandelion.The constituents were isolated and purified by silica gel column chromatography,reversed phase high performance liquid chromatography and semi-preparative high performance liquid chromatography.The structures were elucidated on the basis of physicochemical properties and spectral analysis.RESULTS Six compounds were isolated and identified as palmitic acid （Ⅰ）,caffeic acid （Ⅱ）,ethyl caffeate （Ⅲ）,ethyl 4-hydroxyphenylacetate （Ⅳ）,ferulic acid （Ⅴ） and blumenol A （Ⅵ）.CONCLUSION Compound Ⅵ is isolated from dendelion for the first time.

关 键 词：蒲公英 化学成分 渗漉法 色谱分离 鉴定 

分 类 号：R932[医药卫生—生药学]