枸杞总黄酮提取物对2型糖尿病大鼠血管内皮细胞的保护作用  

Protective effect of total flavonoids of lycium barbarum on vascular endothelial cells in type 2 diabetic rats

作　　者：王伟[1] 尚佳[1] 楚元奎[2] 

机构地区：[1]巴彦淖尔市医院,内蒙古巴彦淖尔015000 [2]宁夏医科大学检验学院,银川750004

出　　处：《重庆医学》2017年第32期4481-4483,共3页Chongqing medicine

基　　金：宁夏自然科学基金资助项目(NZ11116);氯化锂对小鼠糖尿病动脉粥样硬化防治作用的初步研究(XM2011008)

摘　　要：目的研究枸杞总黄酮提取物(TFLB)对链脲佐菌素(STZ)诱导建立的糖尿病(DM)大鼠损伤模型血管内皮细胞保护作用。方法采用STZ诱导建立DM大鼠损伤模型,实验分为正常对照组、DM模型组和TFLB干预组(60、90、120mg/mL)。正常对照组及DM模型组大鼠每天每只予以等量生理盐水灌胃,干预组大鼠以不同浓度TFLB灌胃4周。灌胃满4周后,采用ELISA法测定大鼠血清血管性血友病因子(vWF)、可溶性血栓调节蛋白(sTM)、可溶性血管内皮细胞蛋白C受体(sEPCR)变化情况,摘取胸主动脉组织做HE染色,观察TFLB对DM大鼠血管内皮的保护作用。结果 TFLB干预后,与DM模型组相比较,下调了血管内皮功能相关因子vWF、sTM、sEPCR的水平,差异有统计学意义(P<0.05,P<0.01);随着TFLB剂量的升高,干预组大鼠主动脉内膜增厚程度明显减轻,山峦样突起逐渐减少,外膜未见明显损伤,血管形态排列趋于规整。结论 TFLB可以改善高血糖对血管内皮细胞的损伤,调节血管损伤相关因子含量的改变,延缓DM并发症的进展程度,起到保护心血管系统作用。Objective To study the protective effect of total flavonoids of lycium barbarum（TFLB）on vascular endothelial cells in streptozotocin（STZ）-induced diabetic mellitus（DM）rats injury model.Methods The injury model of DM rats was induced by STZ,and the rats were divided into normal control group,DM model group and TFLB intervention group（60,90 and 120 mg/mL）.Rats in the normal control group and the DM model group were given the same amount of intragastric administration of normal saline every day.The rats in the intervention group were treated with TFLB at different concentrations for 4 weeks.After filling the stomach for 4 weeks,the blood samples were taken from the chest cavity,and the changes of serum vWF,sTM and sEPCR were measured by ELISA.The tissue of the thoracic aorta was removed and stained by HE,and the protective effect of TFLB on the vascular endothelium in DM rats was observed.Results Compared with DM model group,the levels of vascular endothelial function related factors vWF,sTM and sEPCR were down-regulated in the TFLB intervention group,and the difference was statistically significant（P〈0.05,P〈0.01）.In the TFLB intervention group,with the increase of TFLB dose,the intima thickening degree of the rats was significantly reduced;the formation of mountain-like protrusions was gradually decreased;the outer membrane showed no significant damage;the vascular morphology arrangement tended to be regular.Conclusion TFLB can improve the hyperglycemiacaused damage of vascular endothelial cells,regulate the content of vascular injury related factors,delay the progression of DM complications,and protect the cardiovascular system.

关 键 词：枸杞总黄酮 糖尿病 2型 血管内皮细胞 

分 类 号：R285.5[医药卫生—中药学]