静脉血内脂素和胰岛素样生长因子Ⅰ与犊牛初生重相关性研究  

Relationship of Visfatin and IGF-Ⅰ in Venous Blood with Calf Birth Weight

作　　者：傅宏庆[1] 姚志兰[1] 沈留红[2] 肖劲邦 宗佳丽 江涛[2] 朱凯 魏惊鸿 

机构地区：[1]江苏农牧科技职业学院,江苏泰州225300 [2]四川农业大学动物医学院,四川成都611130

出　　处：《云南农业大学学报（自然科学版）》2017年第5期823-829,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：江苏农牧科技职业学院产业发展关键技术创新项目(NSF201604);四川省教育厅重点项目(15ZA0024);四川农业大学双支计划项目(03571303);四川农业大学本科科研兴趣培养计划项目(KY2016062)

摘　　要：【目的】研究奶牛、犊牛和脐静脉血内脂素、IGF-Ⅰ之间及其与犊牛初生重的相关性。【方法】选取规模化养殖场正常分娩奶牛54头,按犊牛初生重划分为A组(≤40 kg,9头)、B组(40~45 kg,25头)和C组(≥45 kg,20头),分别采集分娩奶牛、犊牛及脐静脉血,ELISA法检测血清内脂素、IGF-Ⅰ含量,分析其间的相关性及其与犊牛初生重的相关性。【结果】奶牛静脉血、犊牛静脉血及脐静脉血中均有内脂素、IGF-Ⅰ表达。随犊牛初生重增加,A组犊牛和脐静脉血内脂素显著低于C组(P<0.05),A组和B组犊牛、脐静脉血IGF-Ⅰ含量均分别显著高于C组(P<0.05),各组其余指标间差异均不显著(P>0.05);奶牛、犊牛和脐静脉血中内脂素与IGF-Ⅰ均分别呈极显著正相关(P<0.01);犊牛初生重与奶牛静脉血内脂素、IGF-Ⅰ相关性均不显著(P>0.05),与犊牛静脉血内脂素呈显著正相关(P<0.05),与犊牛和脐静脉血IGF-Ⅰ以及脐静脉血内脂素呈极显著正相关(P<0.01);奶牛静脉血内脂素、IGF-Ⅰ与脐静脉血和犊牛静脉血内脂素、IGF-Ⅰ相关性均分别不显著(P>0.05),而犊牛静脉血内脂素、IGF-Ⅰ与脐静脉血内脂素、IGF-Ⅰ均分别呈极显著正相关(P<0.01)。奶牛静脉血、犊牛静脉血及脐静脉血内脂素、IGF-Ⅰ在公、母犊间差异均不显著(P>0.05)。【结论】奶牛、犊牛和脐静脉血中均有内脂素、IGF-Ⅰ表达;影响犊牛初生重的是犊牛和脐静脉血内脂素、IGF-Ⅰ而非奶牛静脉血内脂素、IGF-Ⅰ;内脂素、IGF-Ⅰ含量在公犊和母犊间差异均不显著。[Purpose] In order to explore the correlation between visfatin,IGF-Ⅰ in cow calf and cord venous blood and calf birth weight（ CBW）. [Method]54 healthy Chinese Holstein cows,which experienced normal delivery,were used in the present study. The cows were grouped according to birthweight of their calves（ group A： ≤40 kg,n = 9; group B： 40-45 kg,n = 25; group C： ≥45 kg,n = 20）. Cow,calf and cord venous blood were collected respectively after delivery. ELISA was used to evaluate the level of visfatin and IGF-Ⅰ in serum,the correlation among them as well as correlation with CBW was analyzed. [Result]Visfatin and IGF-Ⅰ expressed in cow,calf and cord venous blood.With the increase in CBW,the visfatin in calf and cord venous blood of group A were significant higher those of group C（ P 0. 05）,the IGF-Ⅰ in calf and cord venous blood of group A and group B were significant higher than those in group C（ P 0. 05）,and the differences of other indexes were not significant in groups（ P 0. 05）. The visfatin and IGF-Ⅰ in cow,calf and cord venous blood had highly significant positive correlation with each other（ P 0. 01）. CBW had no significant correlation between the expression levels of visfatin and IGF-Ⅰ in cow venous blood（ P 0. 05）,but had significant correlation between visfatin in calf venous blood（ P 0. 05） and had highly significant correlation with IGF-Ⅰ in calf and cord venous blood and visfatin in cord venous blood（ P 0. 01）. There were no significant correlation between the expression levels of visfatin and IGF-Ⅰ in cow venous blood and those in calf and cord venous blood（ P 0. 05）; while visfatin and IGF-Ⅰ in calf venous blood had highly significant positive correlation with those in cord blood venous（ P 0. 01）. Visfatin and IGF-Ⅰin cow,calf and cord in venous blood had no significant differences between the male and the female calf respectively（ P 0. 05）. [Conclusion] There were visfatin and IGF-Ⅰ expressed in cow,calf and cord venous bloo

关 键 词：奶牛 内脂素 胰岛素样生长因子1 脐静脉 犊牛初生重 

分 类 号：S823.1[农业科学—畜牧学]