铁皮石斛多糖提取工艺及优化研究  

Research on Optimizing Extraction Technology of Polysaccharides from Dendrobium officinale

作　　者：黄丽[1] 文凤娟 李桂琼[1] 郭成鑫 熊娇[1] 何丹[1] 周洋帆[1] 贺飞飞 文国松[1] 

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]云南大学农学院,云南昆明650091

出　　处：《云南农业大学学报（自然科学版）》2017年第5期884-888,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：农业科技成果转化资金项目(SQ2013ECF300019)

摘　　要：【目的】考察铁皮石斛多糖提取方法及不同产地多糖含量的差异。【方法】采用正交试验设计筛选料液比、提取时间、提取次数对铁皮石斛多糖提取率的影响。【结果】铁皮石斛多糖提取最佳工艺条件为料液比1∶40,提取时间2 h,提取次数3次;分析云南省不同产地的铁皮石斛多糖含量,结果表明景洪勐龙的含量最高,可达到45.23%。【结论】获得铁皮石斛多糖提取的最佳工艺条件,不同产地的铁皮石斛多糖含量存在较大差异。[Purpose]The purpose of this study as to optimize the extraction protocols of polysaccharides from Dendrobium officinale and the content difference of polysaccharides in D. officinale from different origins. [Method]The extraction efficiency was screened by orthogonal experiment with the ratio of raw materials to solvent,extracting time,and extracting frequency as factors. [Results] The optimal extraction technology was as followis： the ratio of raw materials to solvent was 1∶ 40,the extracting time was 2 h,and extracting frequency was 3 times. According to the content analysis results of polysaccharides in D. officinale from Yunnan Province,where the content of the highest Menglong Jinghong reached 45. 23%. [Conclusion]The optimum technological conditions were obtained for the extraction of polysaccharides from D. officinale,there are some differences in the content of polysaccharide in D. officinale from different habitats.

关 键 词：铁皮石斛 多糖 提取工艺 

分 类 号：R284.2[医药卫生—中药学]