BHK-21细胞不同处理方式对裸鼠致瘤性的影响  

Effects of Different Treatments of BHK-21 Cells on Tumorigenicity in Nude Mice

作　　者：于义娟 秦涛 闵娟娟 漆世华 靖志强 付娅 李晶梅 廖园园 舒银辉 朱薇 徐松 谢红玲 

机构地区：[1]武汉中博生物股份有限公司,武汉430070

出　　处：《中国兽药杂志》2017年第11期28-35,共8页Chinese Journal of Veterinary Drug

摘　　要：为分析BHK-21细胞不同处理方式对裸鼠致瘤性的影响,分别用甲醛、二乙烯亚胺(BEI)、β-丙内酯(BPL)进行灭活和在-20℃进行冻融处理的BHK-21细胞接种裸鼠,通过病理组织形态学观察判定其是否存在致肿瘤的特性。结果显示,甲醛、BEI、BPL、冻融2次或3次分别处理的BHK-21细胞接种裸鼠,对裸鼠致瘤率为零。冻融1次的BHK-21细胞和10~7、10~5和10~3个BHK-21细胞分别接种裸鼠,致瘤率为100%。10个BHK-21细胞接种裸鼠,未见成瘤。试验表明,裸鼠检测BHK-21细胞致瘤性敏感性为10~3个细胞;冻融2次、甲醛、BEI、BPL处理,BHK-21细胞均丧失致瘤性;冻融1次不能使BHK-21细胞丧失致瘤性。For understanding the tumorigenicity of BHK-21 cells, nude mice were injected by BHK-21 cells which inactivated by formaldehyde, diethylenimine （BEI）, β-propiolactone （BPL） as well as BHK-21 cells which freezing- thawing at -20 ℃, the tumor- bearing characteristics were observed by pathomorphologieal changes. The results showed that the tumor morbidity of BHK-21 cells were treated by formaldehyde, BEI, BPL and freezing-thawing for two or three times were zero, 107, 105 , 103 BHK-21 cells can 100% cause the tumors as well as the BHK-21 cells were freezing-thawing only once, no tumors were found when the dose of injection is ten. These results suggested that the sensitivity of tumorigenicity of BHK-21 cells in nude mice was 103 cells, the tumorigenicity of BHK-21 cells can be killed with freezing-thawing for twice, formaldehyde, BEI and BPL; but it still have tumorigenicity in nude mice with freezing-thawing only once.

关 键 词：BHK-21细胞 裸鼠 致瘤性 

分 类 号：Q813[生物学—生物工程]