HPLC法同时测定散风宁嗽糖浆中7种成分  

Simultaneous determination of seven constituents in Sanfeng Ningsou Syrup by HPLC

作　　者：周敏[1] 

机构地区：[1]江苏食品药品职业技术学院药学院,江苏淮安223003

出　　处：《中成药》2017年第11期2301-2304,共4页Chinese Traditional Patent Medicine

基　　金：江苏省自然科学青年基金(BK20140449)

摘　　要：目的建立HPLC法同时测定散风宁嗽糖浆(苦杏仁、化橘红、前胡等)中苦杏仁苷、芸香柚皮苷、柚皮苷、野漆树苷、白花前胡甲素、白花前胡乙素、白花前胡素E的含有量。方法该药物甲醇提取液的分析采用Agilent SB-C18色谱柱(4.6 mm×250 mm,5μm);以乙腈-甲醇(1∶1)-0.1%磷酸为流动相,梯度洗脱;体积流量0.9 m L/min;柱温25℃;检测波长207、283、321 nm。结果 7种成分在各自范围内线性关系良好(r>0.999 0),平均加样回收率96.85%~99.95%,RSD 0.86%~1.66%。结论该方法稳定可靠,可用于散风宁嗽糖浆的质量控制。AIM To establish an HPLC method for the simultaneous content determination of amygdalin,narirutin,naringin,rhoifolin,praeruptorin A,praeruptorin B and praeruptorin E in Sanfeng Ningsou Syrup（Armeniacae Semen Amarum,Citri grandis Exocarpium,Peucedani Radix,etc.）. METHODS The analysis of methanol extract of this drug was performed on a 25 ℃ Agilent SB-C18 column（4. 6 mm × 250 mm,5 μm）,with the mobile phase comprising of acetonitrile-methanol（1 ∶ 1）-0. 1% phosphoric acid flowing at 0. 9 m L/min in a gradient elution manner,and the detection wavelengths were set at 207,283 and 321 nm. RESULTS Seven constituents showed good linear relationships within their own ranges（r 〉 0. 999 0）,whose average recoveries were96. 85%-99. 95% with the RSDs of 0. 86%-1. 66%. CONCLUSION This stable and reliable method can be used for the quality control of Sanfeng Ningsou Syrup.

关 键 词：散风宁嗽糖浆 化学成分 HPLC 

分 类 号：R927.2[医药卫生—药学]