HPLC-ELSD法同时测定川贝罗汉止咳颗粒中7种成分  

Simultaneous determination of seven constituents in Chuanbei Luohan Zhike Granules by HPLC-ELSD

作　　者：李敏[1] 聂建军[2] 魏简汇[1] 郑芝欣[1] 

机构地区：[1]郑州大学附属医院/南阳市中心医院药学部,河南南阳473000 [2]南阳医学高等专科学校第一附属医院药剂科,河南南阳473000

出　　处：《中成药》2017年第11期2304-2307,共4页Chinese Traditional Patent Medicine

摘　　要：目的建立HPLC-ELSD法同时测定川贝罗汉止咳颗粒(川贝母、枇杷叶、桔梗等)中野鸦椿酸、委陵菜酸、山楂酸、科罗索酸、桔梗皂苷E、桔梗皂苷D3、桔梗皂苷D的含有量。方法该药物60%甲醇提取液的分析采用Ultimate XB C18色谱柱(4.6 mm×250 mm,5μm);以乙腈-0.5%醋酸铵为流动相,梯度洗脱;体积流量0.6 m L/min;柱温30℃。结果 7种成分在各自范围内线性关系良好(r>0.999 0),平均加样回收率96.99%~99.78%,RSD0.72%~1.72%。结论该方法准确简便,专属性强,重复性好,可用于川贝罗汉止咳颗粒的质量控制。AIM To establish an HPLC-ELSD method for the simultaneous content determination of euscaphic acid,tormentic acid,crategolic acid,corosolic acid,platycodin E,platycodin D3 and platycodin D in Chuanbei Luohan Zhike Granules（Fritillariae cirrhosae Bulbus, Eriobotryae Folium, Platycodonis Radix, etc.）.METHODS The analysis of 60% methanol extract of this drug was performed on a 30 ℃ thermostatic Ultimate XB C18 column（4. 6 mm × 250 mm,5 μm）,with the mobile phase comprising of acetonitrile-0. 5% ammonium acetate flowing at 0. 6 m L/min in a gradient elution manner. RESULTS Seven constituents showed good linear relationships within their own ranges（r 〉 0. 999 0）,whose average recoveries were 96. 99%-99. 78% with the RSDs of 0. 72%-1. 72%. CONCLUSION This accurate,simple,specific and reproducible method can be used for the quality control of Chuanbei Luohan Zhike Granules.

关 键 词：川贝罗汉止咳颗粒 化学成分 HPLC-ELSD 

分 类 号：R927.2[医药卫生—药学]