HPLC法同时测定交泰丸中6种成分  

Simultaneous determination of six constituents in Jiaotai Pills by HPLC

作　　者：朱立静[1] 宗阳[1] 何书芬[1] 张倩[1] 居文政[1] 

机构地区：[1]南京中医药大学附属医院,江苏南京210029

出　　处：《中成药》2017年第11期2308-2311,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目(81573685);重大新药创制项目(2012ZX09303009-002)

摘　　要：目的建立HPLC法同时测定交泰丸(黄连和肉桂)中6种成分的含有量。方法该药物30%甲醇提取液的分析采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-KH2PO4,梯度洗脱;体积流量0.8 m L/min;柱温30℃;检测波长276 nm。结果表小檗碱、盐酸药根碱、盐酸黄连碱、盐酸巴马汀、盐酸小檗碱、肉桂醛分别在0.64~41.24μg/m L(R2=0.999 9)、0.65~43.76μg/m L(R2=1.000 0)、0.82~52.65μg/m L(R2=0.999 9)、0.79~50.70μg/m L(R2=0.999 9)、3.08~197.20μg/m L(R2=0.999 8)、0.65~41.65μg/m L(R2=0.999 9),平均加样回收率分别为98.06%、102.76%、99.27%、99.75%、96.74%、101.33%,RSD分别为0.56%、0.54%、0.39%、0.55%、0.48%、2.14%。结论该方法准确、灵敏、稳定、重复性好,可用于交泰丸的质量控制。AIM To establish an HPLC method for the simultaneous content determination of six constituents in Jiaotai Pills（Coptidis Rhizoma and Cinnamomi Cortex）. METHODS The analysis of 30% methanol of this drug was performed on a 30 ℃ Agilent ZORBAX SB-C18 column（4. 6 mm × 250 mm,5 μm）,with the mobile phase comprising of acetonitrile-KH2 PO4 flowing at 0. 8 m L/min in a gradient elution manner,and the detection wavelength was set at276 nm. RESULTS Epiberberine,jatrorrhizine hydrochloride,coptisine hydrochloride,palmatine chloride,berberine hydrochloride and cinnamaldehyde showed good linear relationships within the ranges of 0. 64-41. 24 μg/m L（R2=0. 999 9）,0. 65-43. 76 μg/m L（R2= 1. 000 0）,0. 82-52. 65 μg/m L（R2= 0. 999 9）,0. 79-50. 70 μg/m L（R2= 0. 999 9）,3. 08-197. 20 μg/m L（R2= 0. 999 8） and 0. 65-41. 65 μg/m L（R2= 0. 999 9）,whose average recoveries were 98. 06%,102. 76%,99. 27%,99. 75%,96. 74% and 101. 33% with the RSDs of 0. 56%,0. 54%,0. 39%,0. 55%,0. 48% and 2. 14%,respectively. CONCLUSION This accurate,sensitive,stable and reproducible method can be used for the quality control of Jiaotai Pills.

关 键 词：交泰丸 表小檗碱 盐酸药根碱 盐酸黄连碱 盐酸巴马汀 盐酸小檗碱 肉桂醛 HPLC 

分 类 号：R927.2[医药卫生—药学]