21个产地七叶一枝花中皂苷类成分的评价  

Evaluation of saponins in Paris Polyphylla var. chinensis from twenty-one growing areas

作　　者：陈铁柱[1] 文飞燕[1] 张涛[1] 杨玉霞[1] 方清茂[1] 张浩[2] 薛丹[2] 

机构地区：[1]四川省中医药科学院,四川成都610041 [2]四川大学华西药学院,四川成都610041

出　　处：《中成药》2017年第11期2345-2350,共6页Chinese Traditional Patent Medicine

基　　金：中央本级重大增减支项目(2060302);四川省中医药管理局科技专项(2010-46);四川省科技厅应用基础研究(2016JY0081);四川省科技厅科技扶贫专项(2016NFP0073);四川省科技厅科技创新苗子工程项目(2017RZ0051);四川省公益性科研院所基本科研业务专项(2016YSKY0084)

摘　　要：目的对21个产地七叶一枝花Paris Polyphylla Smith var.chinensis(Franch.)Hara中的皂苷类成分进行评价。方法 HPLC法测定偏诺皂苷{偏诺皂苷-3-O-β-D-glu(1→3)[α-L-rha(1→2)]-β-D-glu(PGGR)、重楼皂苷Ⅵ、Ⅶ、H}和薯蓣皂苷(纤细薯蓣皂苷、重楼皂苷Ⅰ、Ⅱ、Ⅴ)的含有量,SPSS 22.0软件进行主成分分析和聚类分析。结果偏诺皂苷为七叶一枝花皂苷的主要种类。PGGR、重楼皂苷Ⅰ、Ⅴ、Ⅶ、H为该药材主要有效成分;7个产地的综合得分大于0,依次为巴中>青川>盐津>通江>南川>崇州>洪雅。21批样品分为4类,与聚类分析结果相似。结论四川巴中、青川、崇州、洪雅、通江,云南盐津,重庆南川适宜栽培七叶一枝花,可保证皂苷类成分的稳定性。AIM To evaluate saponins in Paris Polyphylla Smith var. chinensis（Franch.） Hara from twentyone growing areas. METHODS HPLC was adopted in the content determination of pennogenins { pennogenin-3-O-β-D-glu（1→3） [α-L-rha（1 →2） ]-β-D-glu（PGGR）,polyphyllinsⅥ,Ⅶ,H} and dioscins（gracillin,polyphyllinsⅠ,Ⅱ,Ⅴ）,then SPSS 22. 0 software was used for principal component analysis and cluster analysis.RESULTS Pennogenin was the main kind of Paris Polyphylla var. chinensis saponins,and PGGR,polyphyllinsⅠ,Ⅴ,Ⅶ,H were the main effective constituents of this medicinal material. The comprehension scores of seven growing areas were more than zero,which was in sequence of Bazhong 〉 Qingchuan 〉 Yanjin 〉 Tongjiang 〉 Nanchuan 〉 Chongzhou 〉 Hongya. Twenty-one batches of samples were divided into four types,which was similar to cluster analysis results. CONCLUSION Bazhong,Qingchuan,Chongzhou,Hongya,Tongjiang in Sichuan,Yanjin in Yunnan,and Nanchuan in Chongqing are suitable for cultivating Paris Polyphylla var. chinensis,which can guarantee the stability of saponins.

关 键 词：七叶一枝花 皂苷 产地 HPLC 主成分分析 聚类分析 

分 类 号：R284.1[医药卫生—中药学]