南极普里兹湾海绵共附生放线菌的分离及其次级代谢潜力评估  

Isolation of Antarctic sponge-associated actinobacyeria and evaluation of secondary metabolites biosynthetic potential

作　　者：官颖颖 于雪 张海雷 张宁[1] 李静[1] GUAN Ying-ying;YU Xue;ZHANG Hai-lei;ZHANG Ning;LI Jing(College of Marine Life Sciences,Ocean University of China, Qingdao 266003,Chin)

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《中国海洋药物》2017年第5期1-8,共8页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金青年项目(31200009);极地专项项目(CHINARE2016-01-05)资助

摘　　要：目的通过研究了解南极海域海绵共附生放线菌的多样性以及活性次级代谢产物的合成潜力。方法采用4种分离培养基对南极海绵进行放线菌的分离培养,对分离到的菌株进行抗菌及微藻克生活性的筛选,对活性菌株的进行基于聚酮合酶、非核糖体肽合成酶和卤化酶的基因筛选。结果共分离得到59株海绵共附生放线菌,分布于9个属中,以链霉菌最多。21株具有较好的抗菌活性;6株菌表现出明显的赤潮微藻克生活性;有7株活性菌株至少含2种关键的功能基因,菌株OAct311和OAct408同时含有4种功能基因。结论研究结果揭示了南极海绵共附生放线菌物蕴含丰富的放线菌资源并且有较高的合成活性次级代谢物的潜力,具有深一步研究的价值。Objective To analyze the diversity of actinomyces isolated from Antarctic sponge and to evaluate secondary metabolites biosynthetic potential of these actinomyces.Methods Sponge-associated actinomyces were isolated using 4 media.16 SrDNA sequences of the representatives were amplified and sequenced to analyze phylogenetic diversity of the actinomyces.The antagonistic activity of the sponge-associated actinomyces was screened using modified cross-streak method.The allelopathic activity on redtide microalgae was also tested using growth-inhibition method.A sequence-guided genetic screening strategy was used to evaluate the genetic biosynthetic potential of the active strains.Results 59 strains were isolated and classified into 9 genera.21 strains were screened out with high antagonistic activity and 6 strains showed remarkable allelopathic activity.7 strains were testified to contain at least 2 types of key biosynthetic genes while strain OAct311 and OAct408 contained all the 4 biosynthetic genes tested in this study.Conclusion There were abundant and diverse sponge-associated actinomyces in Antarctic.It is one of the most important sources in searching for new bioactive compounds.

关 键 词：南极 海绵 共附生放线菌 功能基因 

分 类 号：R931[医药卫生—生药学]