藻酸双脂钠联合更昔洛韦在体外抗人巨细胞病毒感染的效果研究（英文）  

Effects of PSS combined with GCV on HCMV infection in vitro

作　　者：王金冬[1] 孙延龙[2] 杜宇[1] 李文通[3] 吴洪娟[4] 王刚[5] 姜子童 阮极盟 刘志军[1] WANG Jin-dong;SUN Yan-long;DU YuI;LI Wen-tong;WU Hong-juan;WANG Gangs;JIANG Zi-tong;RUAN Ji-meng;LIU Zhi-jun(Department of Microbiology;Department of Medicinal chemistry;Department of Pathology;Department of Morphological laboratory;Department of Genetics;Department of Clinical Medicine, Weifang Medical University, Weifang 261053, China)

机构地区：[1]潍坊医学院病原生物学教研室,山东潍坊261053 [2]潍坊医学院药物化学教研室,山东潍坊261053 [3]潍坊医学院病理学教研室,山东潍坊261053 [4]潍坊医学院形态学实验室,山东潍坊261053 [5]潍坊医学院医学遗传学教研室,山东潍坊261053 [6]潍坊医学院临床医学院,山东潍坊261053

出　　处：《中国海洋药物》2017年第5期69-74,共6页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(81471048);山东省自然科学基金项目(ZR2012HL42,ZR2015HL064);山东省教育厅高等学校科技计划项目(J12LK01);潍坊医学院大学生科技创新基金(KX2015015)资助

摘　　要：目的研究藻酸双脂钠(PSS)联合更昔洛韦(GCV)在体外抗人巨细胞病毒(HCMV)感染的作用。方法应用体外细胞培养技术构建HCMV-AD169感染人胚肺成纤维细胞(HELF)模型,对照组采用GCV(50mg·L^(-1))单一药物,实验组采用PSS(100mg·L^(-1))和GCV(50mg·L^(-1))的联合药物,应用两组药物同时对模型进行干预,观察干预后两组的特征性致细胞病变效应(CPE)的变化,同时应用RT-PCR法检测用药后两组HCMV的IE2的表达情况。结果实验组和对照组均可提高感染细胞的存活率,同时降低感染细胞中HCMV IE2的mRNA表达量。显微镜下形态学观察显示,相比对照组,实验组能明显降低药物对HELF细胞的毒性作用,提高细胞存活率。结论 PSS联合GCV具有良好的体外抗HCMV作用,二者具有协同抗病毒作用。Objective To study the effect of ganciclovir（GCV）combined with propylene glycol alginate sodium sulfate（PSS）on human cytomegalovirus（HCMV）infectionin vitro.Methods A human embryonic lung fibroblast（HELF）model of HCMV-AD169 infection was established in vitro.GCV（50 mg·L^-1）alone or in combination with PSS（100 mg·L^-1）was added to HCMV-infected HELF cultures and the characteristic cytopathic effects were assessed.RT-PCR was used to evaluate HCMV IE2（Immediate-Early 2）mRNA expression.Results Both GCV and GCV plus PSS increased the survival rate and decreased the expression of HCMV IE2 mRNA in infected HELFs.The morphological changes observed by light microscopy showed that the survival rate was increased and the toxic effect on the HELFs was weakened in GCV plus PSS group.Conclusion PSS combined with GCV has a good antiHCMV effect in vitro,and these drugs have synergistic antiviral effects.

关 键 词：更昔洛韦 藻酸双脂钠 人巨细胞病毒 

分 类 号：R373[医药卫生—病原生物学] R978.7[医药卫生—基础医学]