蛇床子素对APP/PS1双转基因AD小鼠的治疗作用  

Therapeutic effect of osthole on APP/PS1 double transgenic AD mice

作　　者：王雅萌 教亚男 闫宇辉[1] 李少恒[1] 宋捷[1] 王玉莹 李秀丽 杨静娴[1] 

机构地区：[1]辽宁中医药大学药理教研室,大连116000

出　　处：《中国新药杂志》2017年第21期2573-2577,共5页Chinese Journal of New Drugs

基　　金：国家自然科学基金资助项目(81173580)

摘　　要：目的:研究蛇床子素对APP/PS1双转基因阿尔茨海默病(AD)小鼠的治疗作用。方法:将6月龄的雄性APP/PS1双转基因小鼠随机分为模型组与蛇床子素组,同月龄的雄性C57BL/6小鼠作为阴性对照组;水迷宫法检测各组小鼠的学习记忆能力;HE及尼氏染色法检测小鼠脑组织病理损伤情况;免疫荧光组织化学法检测各组小鼠脑组织中Aβ蛋白的表达。结果:与模型组相比,蛇床子素组可以改善AD小鼠的学习记忆能力,减轻小鼠脑组织病理损伤,降低小鼠脑组织内Aβ蛋白表达。结论:蛇床子素对APP/PS1小鼠具有一定的治疗作用,其可以减轻脑内病理损伤,提高小鼠的学习与记忆能力,可能与降低Aβ表达有关。Objective： to study the therapeutic effect of osthole on APP/PS1 double transgenic mouse model of AD. Methods： the six months old APP/PS1 double transgenic male mice were randomly divided into model group and osthole group,with the same months old C57 BL/6 male mice as negative control group; after six weeks,the ability of learning and memory of mice were detected by Morris water maze; HE and Nissl staining in mouse brain injury; the expression of Aβ protein in mice brain was detected by immunohistochemistry technique. Results：compared with the model group,the osthole group could improve the learning and memory ability of APP/PS1 mice,reduce the pathological damage of brain tissue,and downregulate the expression of Aβ protein in brain tissue of mice. Conclusion： the therapeutic effect of osthole on APP/PS1 double transgenic mice,it reduce the brain pathological injury and improve the ability of learning and memory in mice,which may be related to the expression decrease of Aβ.

关 键 词：蛇床子素 阿尔茨海默病 学习记忆能力 APP/PS1双转基因小鼠 

分 类 号：R932[医药卫生—生药学]