仙灵骨葆胶囊干预老龄骨质疏松模型大鼠骨力学特性的变化  

Mechanical properties of a osteoporotic rat model after treated with Xianling Gubao Capsule

作　　者：李鹏[1] 李新颖[2] 李正伟[3] 李亚军[4] 

机构地区：[1]吉林大学南岭校区工程力学系,吉林省长春市130022 [2]吉林大学中日联谊医院超声科,吉林省长春市130033 [3]吉林大学第二医院骨科,吉林省长春市130026 [4]吉林大学数学学院,吉林省长春市130028

出　　处：《中国组织工程研究》2017年第32期5170-5176,共7页Chinese Journal of Tissue Engineering Research

基　　金：吉林省科技发展计划资助项目(20110492)~~

摘　　要：背景:以压缩、弯曲力学性能指标评估仙灵骨葆胶囊等多种药物治疗老龄雌性骨质疏松模型动物的效果目前鲜有报道。目的:对以仙灵骨葆胶囊、阿仑膦酸钠、钙尔奇、甲状旁腺激素(3-34).牛干预治疗的骨质疏松模型大鼠股骨胫骨进行压缩、弯曲实验,确定各种药物对骨质疏松的治疗效果。方法:以去双侧卵巢的方法建立雌性大鼠骨质疏松模型,分别以仙灵骨葆胶囊、阿仑膦酸钠、钙尔奇、甲状旁腺激素(3-34).牛进行干预治疗,给药20周。给药20周后,以腹主动脉放血法处死各组大鼠,解剖取各组大鼠左、右侧股骨进行压缩实验,取大鼠左、右侧胫骨进行弯曲实验。结果与结论:(1)股骨压缩实验:模型组大鼠股骨压缩弹性应力、弹性载荷、弹性应变、弹性位移、最大载荷最大应变、最大应力、最大位移、弹性模量均显著低于正常对照组、阿仑膦酸钠组、仙灵骨葆胶囊组、甲状旁腺激素(3-34).牛组(P<0.05),钙尔奇组拉伸与弯曲实验各项力学性能指标与模型组差异无显著性意义(P>0.05);仙灵骨葆胶囊组大鼠股骨最大载荷、最大位移、最大应变、最大应力与正常对照组差异无显著性意义(P>0.05);(2)胫骨弯曲实验:模型组大鼠胫骨弯曲实验最大载荷、最大应力、最大弯矩、最大应变、抗弯截面模量均显著低于正常对照组、仙灵骨葆胶囊组、阿仑膦酸钠组、甲状旁腺激素(3-34).牛组(P<0.05),钙尔奇组各项弯曲力学性能指标与模型组差异无显著性意义(P>0.05);仙灵骨葆胶囊组大鼠各项弯曲力学性能指标与正常对照组差异无显著性意义(P>0.05);(3)综上,模型组大鼠因骨质疏松导致股骨的压缩、胫骨的弯曲力学特性发生了改变。骨质疏松大鼠模型以仙灵骨葆胶囊、阿仑膦酸钠、甲状旁腺激素(3-34).牛干预治疗后股骨的压缩、胫骨的弯曲力学特性均有一定恢复,以仙灵骨葆胶囊干预治疗BACKGROUND： The effect of Xianling Gubao Capsule for the elderly female osteoporotic animal models assessed bycompression and bending mechanical properties is little reported.OBJECTIVE： To investigate the curative efficacy of Xianling Gubao Capsule, alendronate sodium, calcium carbonateand parathyroid hormone （3-34） for an osteoporotic rat model through compressive and bending tests at the femur.METHODS： The rat models of osteoporosis were established by bilateral ovariectomy, and were then subjected to thetreatment of Xianling Gubao Capsule, alendronate sodium, calcium carbonate and parathyroid hormone （3-34）,respectively, for 20 weeks. Afterwards, the rats were killed by cutting the abdominal aorta, and then the bilateral femurswere removed to undergo compressive and bending tests.RESULTS AND CONCLUSION： The compressive test： the elastic stress, elastic load, elastic strain, elastic displacement,maximum load, maximum strain, maximum stress, maximum displacement and elastic modulus in the model group weresignificantly lower than those in the blank control, Xianling Gubao Capsule, alendronate sodium and parathyroidhormone （3-34） groups （P 〈 0.05）. The maximum load, maximum strain, maximum stress, and maximum displacementdid not differ significantly between Xianling Gubao Capsule and blank control groups （P 〉 0.05）. All indexes in thecompressive and bending tests showed no significant differences between model and calcium carbonate groups （P 〉0.05）. Bending test： the maximum load, maximum stress, maximum strain, maximum bending moment and bendingsection modulus in the model group were significantly lower than those in the blank control, Xianling Gubao Capsule,alendronate sodium and parathyroid hormone （3-34） groups （P 〈 0.05）. The bending mechanical indexes showed nosignificant differences between Xianling Gubao Capsule and blank control groups （P 〉 0.05）. To conclude, aftercompressive and bending tests, Xianling Gubao Capsule, alendronate sodium, and parat

关 键 词：组织构建 骨组织工程 骨质疏松 动物模型 仙灵骨葆胶囊 阿仑膦酸钠 甲状旁腺激素(3-34).牛 药物治疗 压缩 弯曲 

分 类 号：R318[医药卫生—生物医学工程]