电针督脉经穴联合成体干细胞移植策略在修复脊髓损伤中的研究进展  

Study on Combination Strategy of Governor Vessel Acupoints Electroacupuncture and Adult Stem Cell Transplantation in Repaired Spinal Cord Injury

作　　者：曾园山 

机构地区：[1]中山大学中山医学院组织胚胎学教研室,广东广州510080 [2]广东省脑功能与脑疾病重点实验室,广东广州510080 [3]中山大学脊髓损伤研究所,广东广州510120

出　　处：《中山大学学报（医学科学版）》2017年第6期801-807,847,共8页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金面上项目(30472132;30973721;8167150879;81774397)

摘　　要：本文以电针督脉经穴、内源性神经营养因子表达、移植的成体干细胞源性神经元与脊髓宿主神经元环路整合修复脊髓损伤为主线,概述了电针督脉经穴联合成体干细胞移植改善脊髓损伤组织微环境重建神经元传导通路机制—电针督脉经穴刺激全横断脊髓损伤或脊髓脱髓鞘损伤的大鼠脊膜支传入神经纤维将信息传入脊髓,激活脊髓组织细胞合成和分泌神经营养素-3(NT-3),介导表达NT-3受体Trk C的外源性神经干细胞(NSC)和骨髓间充质干细胞(MSC)在全横断脊髓损伤/移植处或脊髓脱髓鞘损伤/移植处存活、分化和迁移,替换和保护受损伤的宿主神经元、改善受损伤组织微环境、促进神经纤维再生及其髓鞘形成、改善大脑皮层运动诱发电位以及瘫痪肢体的运动功能。On the basis of an outline on Governor Vessel acupoints electroacupuncture,endogenous neurotrophic factor expression,grafted adult stem cell-derived neurons integrating to spinal host neuron circuit for repairing spinal cord injury,it is summarized that mechanism of a combination of Governor Vessel acupoints electroacupuncture and grafted adult stem cells improving the tissue microenvironment of spinal cord injury to reestablish neuron conduction pathway. Governor Vessel acupoints electroacupuncture stimulate meningeal branch of spinal nerve in rat spinal cord transected or demyelinated. The stimulation message is input into spinal cord through afferent nerve,which activates spinal cord cells synthesizing and secreting neurotrophin-3(NT-3). The NT-3 can promote the survival,differentiation and migration of exogenous neural stem cells(NSC)and bone marrow mesenchymal stem cells(MSC)expressing tyrosine kinase receptor C(Trk C)at the injury/graft site or demyelination/graft site in the spinal cord transected or demyelinated. The combined therapeutic strategy may replace and protect host neurons injured,perfect tissue microenvironment injured,promote nerve fiber regenerating,and improve motor evoked potential of cerebral cortex and motor function of paralysis limbs.

关 键 词：电针 督脉经穴 神经营养素-3 神经干细胞 骨髓间充质干细胞 移植 脊髓损伤 脊髓脱髓鞘 

分 类 号：R245.97[医药卫生—针灸推拿学]