基于SSR标记分析贵州50个玉米品种的杂合位点  

Heterozygous Locus of 50 Local Hybrid Maize Varieties Based on SSR Marker Analysis in Guizhou

作　　者：王伟[1] 关琦[2,3] 宋欣 杨文鹏 王明春[1] 

机构地区：[1]贵州省农业科学院旱粮研究所,贵州贵阳550006 [2]贵州大学农学院,贵州贵阳550025 [3]沈阳市翔宇中学,辽宁沈阳110000 [4]威海火炬高技术产业开发区农业经济发展局,山东威海264209

出　　处：《西南农业学报》2017年第11期2417-2424,共8页Southwest China Journal of Agricultural Sciences

基　　金：贵州省科技计划项目[黔科合重大专项字(2013)6022号;黔科合NY(2014)3020];贵州省农业科学院院专项[黔农科院院专项(2014)008];贵州省农业科学院自主创新专项[黔农科院自主创新科研专项字(2014)006];贵州省科研机构服务企业行动计划项目[黔科合服企(2014)4002];贵州省产业技术体系项目(GZCYTX2013-0701)

摘　　要：【目的】为推进贵州玉米种质资源的有效利用、遗传改良以及新品种的选育。【方法】用多态性丰富的85个SSR标记,选取50个来自贵州省内已审定的玉米品种进行分析。【结果】(1)共检测出471个SSR等位基因,SSR位点的等位基因数平均为5.54个,PIC值平均为0.58,表明所用SSR标记具有丰富的遗传多态性。(2)umc2048、bnlg1885、bnlg1083、bnlg2144、phi092、umc2094、umc1726、nc005、umc1491、umc1619和bnlg2228等11个SSR位点的He和Ho差值较高(>0.2),受人为选择和近交等的影响较大。(3)杂合位点的品种数占总品种数的比值>0.5的SSR标记有47个,其中比值>0.8的SSR标记位点有8个。(4)第1染色体的20~55 cM区段,第4染色体的380~590 cM区段,第5染色体的430~495 cM区段,第6染色体的25~40和40~90 cM 2个区段,第7染色体的395~505 cM区段,第8染色体的75~130 cM区段和第9染色体的60~185 cM区段,是杂合位点的重要富集区域。【结论】这些杂合位点富集区域对贵州50个玉米杂交种的杂种优势贡献较大,富集区域含有的优良基因,有利于杂种优势的基因组分子标记辅助选择。【 Objective】This study aimed to improve effective utilization, genetic improvement and variety breeding of maize germplasm re-sources in Guizhou.【 Method】50 approved local maize varieties in Guizhou are analyzed by 85 SSR markers with abundance polymorphism.【 Result】（ i） 471 SSR alleles are detected and the average value and PIC of alleles on SSR loci 5. 54/locus and 0？ 58/locus, which indicatesthat the used SSR markers have the abundant genetic polymorphism. （ii） The difference in He and Ho among 11 SSR loci of umc2048 , bn- lgl885 , bnlgl083 , bnlg2144, phi092, umc2094, umcl726 , nc005 , umcl491, umcl619 and bnlg2228 is high （ 〉0.2） , which indicates the He and Ho are influenced by artificial selection and inbreeding greatly. （iii） There are 47 SSR marker loci with 〉0.5 ratio of varieties with heterozygous locus in total varieties. Of which, the ratio of 8 SSR marker loci is 〉 0. 8. （iv） The segments of 20 - 55 cM on Chromo-some 1,380 - 590 cM on Chromosome 4 ,430 -495 cM on Chromosome 5 , 25 -40 cM and 40 - 90 cM on Chromosome 6 , 395 - 505 cM onChromosome 1,15- 130 cM on Chromosome 8 and 60 - 185 cM on Chromosome 9 are the important enrichment areas of heterozygous loci.【 Conclusion】 The enrichment areas of heterozygous loci have a great contribution to heterosis of 50 hybrid maize varieties in Guizhou. The enrichment enrichment areas with good genes are beneficial to genome marker-assisted selection （MAS） in heterosis of maize.

关 键 词：SSR 杂合位点 杂交种 玉米 

分 类 号：S513[农业科学—作物学]