阳江天然高本底辐射地区居民端粒长度初步研究  

Preliminary research on telomere length of residents in high level natural background radiation area Yangjiang of China

作　　者：李小亮[1] 李坤[2] 孙全富[1] 雷淑洁[1] 赵玉倩 刘建香[1] 谭光享[3] 邹剑明[3] Li Xiaoliang;Li Kun;Sun Quanfu;Lei Shujie;Zhao Yuqian;Liu Jianxiang;Tan Guangxiang;Zou Jianming(Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Belting 100088, China;Department of Nuclear Medicine, Shandong Qianfoshan Hospital, Jinan 250014, China （Li K;Guangdong Prevention and Treatment Center for Occupational Diseases, Guangzhou 510300, China)

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室,北京100088 [2]山东省千佛山医院核医学科,济南250014 [3]广东省职业病防治院,广州510300

出　　处：《中华放射医学与防护杂志》2017年第11期853-857,共5页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的 探索长期低剂量电离辐射对人端粒长度的影响。方法 在阳江市天然放射性高本底地区和恩平市对照地区，采用配额抽样的方法，各选择40名55岁以上、年龄匹配的女性作为高本底组和对照组，采集其外周静脉血，分离基因组DNA，采用实时定量聚合酶链式反应，测量其端粒的相对长度。两组端粒长度均数的比较，对数据取平方根后进行t检验。调查对象按照年龄分为55~、60~、65~和70~岁4组，按照体质量指数（BMI）分为〈18.5、18.5~、24~和28~kg/m2 4组，调整年龄与BMI因素，将全部调查对象的端粒长度与累积受照剂量进行多元线性回归分析。将全部调查对象分为端粒较长组（≥ 2）和较短组（〈2），对端粒长度与累积受照剂量进行Logistic回归分析。结果 高本底组与对照组平均累积受照剂量分别为（169.52±27.43）和（47.52±6.50） mSv。高本底组端粒长度为1.98±1.25，低于对照组的2.69±1.44，差异有统计学意义（t=2.24，P〈0.05）。多元线性回归分析显示，累积剂量对端粒长度的影响无统计学意义（P〉0.05）。对端粒长度与累积受照剂量关系进行Logistic回归分析，累积受照剂量的OR值为0.992，95%可信区间为0.985~0.999，接近于1，端粒长度与受照剂量呈负相关关系，但效应不明显。结论 未发现居民端粒长度与累积受照剂量明显的剂量效应关系，但长期低剂量电离辐射可能会导致人端粒长度变短。Objective To explore the effect of long-term low dose ionizing radiation on telomere length in adults. Methods Forty female residents aged more than 55 years old from high level natural background radiation area in Yangjiang city and forty age-matched female residents from control area in Enping city were selected by quota sampling as high background group and control group, respectively. Genomic DNA was isolated from their peripheral blood. Telomere length was determined using real time q-PCR. The t-test was used to compare the square roots of the means of two groups. The individuals were divided into four groups based on ages （55-, 60-, 65-and ≥ 70） and four groups based on BMI （〈18.5, 18.5-23.99, 24.0-27.99 and ≥ 28.0）. After adjusting age and BMI, multivariate linear regression analysis was performed to study the relationship between telomere length and cumulative exposure dose. The individuals were divided into longer telomere length group （≥ 2） and shorter telomere length group （〈2）. Logistic regression analysis was performed to study the relationship between telomere length and cumulative exposure dose. Results The average cumulative dose was（169.52±27.43）mSv for high background group and（47.52±6.50）mSv for control group. The telomere length of high background group was（1.98±1.25）, shorter than that of control group （2.69±1.44） with statis-tically significant difference（t=2.24, P〈0.05）. The multivariate linear regression analysis revealed that the effect of cumulative dose on telomere length was not significant （P〉0.05）. Association between telomere length and cumulative dose was explored through Logistic regression, and odds ratio was taken as 0.992（95% CI, 0.985-0.999）. There was a weak inverse association between telomere length and cumulative dose, because the odds ratio （OR） was very close to 1. Conclusions No obvious dose-effect relationship between telomere length of residents and cumulative radiation doses was found. But the long-ter

关 键 词：端粒长度 低剂量辐射 高本底地区 

分 类 号：R144[医药卫生—公共卫生与预防医学]