贵州省4个县2013-2014年发热出疹性疾病病原谱的研究  

Pathogenic spectrum of rash and fever illness in 4 counties of Guizhou province,2013-2014

作　　者：吴升伟[1] 唐小敏[1] 戴丽芳[1] 杜雯[1] 叶绪芳[1] Wu Shengwei;Tang Xiaomin;Dai Lifang;et al(Guizhou Provincial Center for Disease Con- trol and Prevention, Guiyang 550004, Guizhou, Chin)

机构地区：[1]贵州省疾病预防控制中心,贵州贵阳550004

出　　处：《中国疫苗和免疫》2017年第5期517-520,525,共5页Chinese Journal of Vaccines and Immunization

基　　金：贵州省科学技术基金(黔科合J字[2014]2106号);贵州省传染病预防与控制人才基地资金资助(RC3D1402)

摘　　要：目的分析贵州省2013年8月-2014年7月发热出疹性疾病(Rash and fever illness,RFI)病原谱的构成特征。方法收集贵州省4个项目县363例RFI病例病原标本,采用实时荧光定量聚合酶链反应(RT-PCR)、聚合酶链反应(PCR)及细菌/病毒分离鉴定等方法检测麻疹病毒(MV)、风疹病毒(RV)、肠道病毒(EV)、EB病毒、水痘-带状疱疹病毒(VZV)、人类小DNA病毒B19、人疱疹病毒6型(HHV-6)、伤寒/副伤寒杆菌。结果 RFI监测病例的病原标本阳性率为20.94%(76/363)。阳性株主要为EV(80.26%)、VZV(11.84%)、MV(5.26%)。在61株EV中,EV71型(EV71)、柯萨奇病毒A16型(CA16)、其他EV分别占21.31%、21.31%和55.74%。VZV基因型为J型。MV基因型为H1a亚型。结论 EV、VZV和MV是RFI的主要病原,开展相应的疫苗接种是控制RFI的主要措施。本研究建立了贵州省RFI实验室监测技术平台。Objective To analyze the pathogenic spectrum of rash and fever illness （RFI） from August 2013 to July 2014 in Guizhou province. Methods We collected pathogenic specimens of 363 RFI cases in 4 counties of Guizhou, and detected measles virus （MV）, rubella virus （RV）, enterovirus （EV）, epstein- barr virus （EBV）, varicella zoster virus （VZV）, parvovirus B19, human herpesvirus 6 （HHV-6）, and ty- phoid and paratyphoid bacillus by reverse transcription quantitative polymerase chain reaction （RT-PCR）, polymerase chain reaction （PCR）, and bacterium/virus isolation and identification. Results The positive identification rate of pathogens among RFI cases was 20. 94% （76/363）. The most commonly-identified viruses were EV （80. 26% ）, VZV （11.84%）, and MV （5.26%）. Among the 61 EV strains, enterovirus 71 （EV71）, Coxsackievirus A6 （CA16）, and other EVs accounted for 21.31%, 21.31% and 55. 74%, respectively. The genotype for VZV was J, and for MV was Hla. Conclusions EV, VZV and MV were the main pathogens of RFI cases. Vaccination is the main measure to control these RFIs. This project established a technical platform for RFI laboratory monitoring in Guizhou.

关 键 词：发热出疹性疾病 病原谱 肠道病毒 水痘-带状疱疹病毒 麻疹病毒 

分 类 号：R373.11[医药卫生—病原生物学]