裙带菜多糖硫酸酯诱导人肝癌HepG-2细胞凋亡机制研究  

Apoptotic Mechanisms of Human Liver Cancer HepG-2 Cells Induced by Undaria pinnatifida Sulfated Polysaccharides

作　　者：杨波[1,2] 王福玲[1,2] 季宇彬[2] 耿豪滨 伊亚彤 刘丽 江蔚新[1] 赵威[1] 

机构地区：[1]哈尔滨商业大学药学院,哈尔滨150076 [2]哈尔滨商业大学生命科学与环境科学研究中心,国家教育部抗肿瘤天然药物工程研究中心,药物研究所博士后科研工作站,哈尔滨150076

出　　处：《中国药学杂志》2017年第21期1909-1913,共5页Chinese Pharmaceutical Journal

基　　金：黑龙江省教育厅面上项目资助(12521134);国家级大学生创新训练计划项目资助(201610240025)

摘　　要：目的探讨裙带菜多糖硫酸酯S-UPPSⅠB体外诱导人肝癌Hep G-2细胞凋亡的作用及机制。方法采用四甲基偶氮唑蓝(MTT)法检测S-UPPSⅠB对Hep G-2细胞增殖作用的影响;激光共聚焦显微镜测定Hep G-2细胞内[Ca^(2+)]i水平;流式细胞仪测定细胞凋亡率及Bcl-2、Bax、Cyt-c、p53的表达;Caspase-3,-9试剂盒检测蛋白表达。结果 S-UPPSⅠB抑制Hep G-2细胞的IC_(50)值为50.09μg·m L^(-1),随着给药剂量的增加,凋亡率亦增加,并且对Ca^(2+)及其通路的相关蛋白有明显的调节作用,其中[Ca^(2+)]i的水平及Cyt-C、Caspase-3,-9、p53的表达均有显著增加(P<0.05),Bcl-2/Bax比值降低。结论裙带菜多糖硫酸酯S-UPPSⅠB可显著抑制人肝癌Hep G-2细胞增殖,并可通过启动线粒体途径诱导Hep G-2细胞发生凋亡。OBJECTIVE To investigate the mechanism of human liver cancer HepG-2 cells apoptosis induced by Undaria pinnat- ifida sulfated polysaccharides S-UPPS I B. METHODS The anti-proliferation effects of S-UPPS I B on HepG-2 cells was by MTT as- say. [ Ca2 ＋ ] i in HepG-2 cells was detected by laser cofocal scaning microscopy （LCSM）. The apoptosis rate and protein expression level of Bcl-2, Bax, Cyt-C and Io53 were detected by flow cytometry （FCM）. Caspase Assay Kit was used to detected the activities of Caspase-3 and -9. RESULTS S-UPPS 1 B could inhibit the proliferation of HepG-2 cells and the IC50 was 50. 09 μg mL-1. With the increase of drug delivery dosage, apoptosis rate also increased, and the significant regulating effects of S-UPPS I B on Ca2＋ and its associated channel proteins were observed. The [ Ca2＋ ] i level, the protein expression level of Cyt-c, p53 and the activities of Caspase- 3 and -9 were all increased remarkably （ P 〈 0. 05 ）. The ratio of Bcl-2 to Bax was reduced. CONCLUSION Undaria pinnatifida sulfated polysaccharides S-UPPS I B can effectively inhibit the proliferation of human liver cancer HepG-2 cells by inducing apoptosis of HepG-2 cells through mitochondrial pathway.

关 键 词：裙带菜 多糖硫酸酯 人肝癌HEPG-2细胞 凋亡 

分 类 号：R965[医药卫生—药理学]