移植到裸鼠体内的糖尿病患者外周血内皮祖细胞再内皮化能力的观察  

Study on the reendothelialization of EPCs in diabetic patients

作　　者：陈文群 王燕国 吴青[3] 王治义 徐寒松[3] 

机构地区：[1]安顺市人民医院疼痛科,561000 [2]内黄县中医院肾内科 [3]贵阳中医学院第二附属医院内分泌科 [4]贵阳中医学院第二附属医院肾内科

出　　处：《中国糖尿病杂志》2017年第11期1021-1026,共6页Chinese Journal of Diabetes

基　　金：国家自然科学基金(30960491);贵州省优秀科技教育人才省长基金[黔省专合字(2009)84号]

摘　　要：目的探讨糖尿病患者外周血内皮祖细胞(EPCs)体内再内皮化能力的变化。方法培养、鉴定EPCs;构建裸鼠颈动脉内膜损伤模型。分组:健康组移植健康人EPCs,糖尿病组移植糖尿病患者EPCs,PBS对照组移植PBS。显微镜下观察伊文斯蓝染色损伤内膜再内皮化的情况,HE染色观察损伤内膜与中膜的面积的厚度,借助Image-Pro Plus 5.0图像分析系统计算再内皮化率及内膜/中膜比值。结果糖尿病组内膜损伤修复面积(23.78±1.95)%小于健康组(35.93±4.37)%(P<0.05),内膜/中膜比值糖尿病组(74.50±6.80)%大于健康组(59.40±5.00)%(P<0.05)。结论糖尿病患者EPCs体内再内皮化及抑制内膜增生能力降低,可能是其血管内皮损伤及内皮修复能力下降的重要机制。Objective To observe the reendothelialization of peripheral blood endothelial progenitor cells（EPCs）in diabetic patients. Methods EPCs are cultured and identified.Carotid artery endothelium injury model was constructed in nude mice.Healthy nude mice accepted the transplantation of EPCs from healthy people;diabetic mice were transplanted with EPCs from DM patients;PBS control group was transplanted with PBS.Under the stereoscopic microscope,Evans Blue staining was used to observe the reendothelialization of the damaged intima,HE dyeing was used to observe the thickness and area of the damaged carotid intima-media.The reendothelialization rate and the ratio of intima/media area were calculated by Image-Pro Plus 5.0 image analysis system. Results The area of intimal repair in diabetic nude mice（23.78±1.95）% was less than that in healthy group（35.93±4.37）%（P〈0.05）.The area ratio of intima/media in healthy group（59.40±5.00）% was less than that in diabetes group（74.50±6.80）%（P〈0.05）. Conclusion The reduced reendothelialization of EPCs and the increased intimal hyperplasia may be an important mechanism for vascular endothelial injury and endothelial repair difficulty in diabetic patients.

关 键 词：内皮祖细胞 糖尿病 再内皮化 颈动脉内膜损伤模型 裸鼠 

分 类 号：R587.2[医药卫生—内分泌]