艾灸预处理对急性胃黏膜损伤大鼠胃组织中TGF-α、PCNA含量的影响  

Effect of moxibustion pretreatment on the content of transforming growth factor-α and proliferating cell nuclear antigen in gastric tissue in rats with acute gastric mucosal injury

作　　者：杨青[1] 舒文娜 雷毅军[1] 蒋学余 邹逸凡 李丹[1] 常小荣[1] 彭艳[1] 刘密[1,2] YANG Qing;SHU Wen - na;LEI Yi - jun;JIANG Xue - yu;ZOU Yi - fan;LI Dan;CHANG Xiao- rong;PENG Yan;LIU Mi(Hunan University of Chinese Medicine, Changsha 410208, Hunan, China;Yueyang Hospital Affiliated to Hunan University of Chinese Medicine ,Yueyang 414000 ,Hunan, China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南中医药大学附属岳阳医院,湖南岳阳414000

出　　处：《湖南中医杂志》2017年第10期151-153,共3页Hunan Journal of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划项目(编号:2015CB554502);国家自然科学基金资助项目(编号:81202770;81574082);高等学校博士学科点专项科研基金新教师类资助课题(编号:20124323120002);湖南省自然科学基金项目(编号:13JJ6060);湖南省优秀博士学位论文获奖作者科研资助项目(编号:YB2013B037);湖南省教育厅资助科研项目(编号:14B129;14B128);2013年湖湘青年科技创新创业平台项目;2013年湖南省高层次卫生人才"225"工程培养项目;湖南中医药大学附属岳阳医院推拿科国家临床重点专科;推拿学国家中医药管理局重点学科资助

摘　　要：目的:观察艾灸预处理对无水乙醇灌胃所致急性胃黏膜损伤大鼠胃组织中转化生长因子-α(TGF-α)、增殖细胞核抗原(PCNA)含量的影响。方法:将48只SD大鼠随机分成4组(空白组、模型组、艾灸穴位组、艾灸对照点组),艾灸穴位组选取足三里穴、中脘穴,艾灸对照点组则选取足三里穴、中脘穴旁开1cm处的对照点,预处理8d后,空白组予0.9%氯化钠注射液灌胃,其余3组予无水乙醇灌胃,1h后取材,光镜下观察各组胃黏膜组织形态学变化,免疫组化法检测胃黏膜TGF-α及PCNA的含量。结果:与空白组比较,模型组大鼠胃黏膜中TGF-α、PCNA的含量明显增高(P<0.01或P<0.05);与模型组比较,艾灸穴位组大鼠胃黏膜中TGF-α、PCNA的含量较高(P<0.01或P<0.05);与艾灸穴位组比较,艾灸对照点组大鼠胃黏膜TGF-α、PCNA的含量较低(P<0.01或P<0.05)。结论:艾灸预处理通过增强急性胃黏膜损伤大鼠胃黏膜中TGF-α、PCNA的表达,促进胃黏膜的增殖修复,且这一过程具有一定的穴位特异性。Objective： To investigate the effect of moxibustion pretreatment on the content of transforming growth factor -α （TGF -α）and proliferating cell nuclear antigen （PCNA）in gastric tissue in rats with acute gastric mucosal injury induced by anhydrous ethanol given by gavage. Methods ： A total of 48 Sprague - Dawley rats were randomly divided into blank group, model group, aeupoint moxibustion group, and control point moxibustion group. The rats in the acupoint moxibustion group were treated with moxibustion at Zusanli and Zhongwanaeupoints, while those in the control point moxibustion group were treated with moxibustion at the points at I cm beside Zusanli and Zhongwanacupoints. After 8 days of pretreatment,the rats in the blank group were given 0.9% sodium chloride injection by gavage,and those in the other three groups were given anhydrous ethanol by gavage. Samples were collected I hour later;the histomorphologicalchanges of the gastric mucosa were observed under a light microscope, and immunohistochemistry was used to measure the content of TGF -α and PCNA in the gastric mucosa. Results：Compared with the blank group, the model group had significant increases in the content of TGF -α and PCNA in the gastric mucosa（P 〈 0. 01 or P 〈 0.05 ） ;compared with the model group, the acupoint moxibustion group had significantly higher content of TGF-α and PCNA in the gastric mucosa（ P 〈 0. 01 or P 〈 0. 05 ） ;compared with the acupoint moxibustion group, the control point moxibustion group had significantly lower content of TGF-α and PCNA in the gastric mucosa（P 〈0. 01 or P 〈 0. 05）. Conclusion ： Moxibustion pretreatment enhances the expression of TGF - α and PCNA in the gastric mucosa in rats with acute gastric mucosal injury and thus promotes the proliferation and repair of the gastric mucosa. This process has a certain degree of acupoint specificity.

关 键 词：艾灸预处理 胃黏膜损伤 TGF-Α PCNA 实验研究 

分 类 号：R285.5[医药卫生—中药学]