骨髓间充质干细胞不同途径输注对阿霉素肾病大鼠AQP1、AQP2表达的影响  

Influence of infusion of mesenchymal stem cells by different routes on the expression of AQP1 and AQP2 in rats with adriamycin-induced nephropathy

作　　者：韩磊[1] 张文卿 孙寰[1] 陆发承 简远熙 邢艺苑 王家平[1] 

机构地区：[1]昆明医科大学第二附属医院放射科,650101

出　　处：《介入放射学杂志》2017年第11期1015-1019,共5页Journal of Interventional Radiology

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项项目(2017FE467);云南省中青年学术和技术带头人后备人才项目(2017HB037)

摘　　要：目的评估骨髓间充质干细胞(MSC)分别通过肾动脉和尾静脉移植方式对大鼠慢性肾病(CKD)模型肾损伤修复的疗效,比较不同移植方式对CKD大鼠水通道蛋白(AQP)1、AQP2表达差异的影响。方法实验取50只大鼠,2只制备骨髓MSC;36只经尾静脉输注阿霉素构建CKD模型,随机分为阿霉素CKD(A-C)组(n=12)、骨髓MSC经肾动脉输注(M-A)组(n=12)、骨髓MSC经尾静脉输注(M-V)组(n=12);另12只为正常对照(N)组。末次骨髓MSC移植隔1周后检测大鼠24 h尿量、24 h尿蛋白定量、血清钠及血清白蛋白,免疫组化检测肾组织AQP1、AQP2表达。结果与A-C组相比,M-V组、M-A组大鼠血清白蛋白及24 h尿量均增高(P<0.05),24 h尿蛋白及血清钠水平均降低(P<0.05),M-A组24 h尿蛋白较M-V组降低更明显(P<0.05)。M-V组、M-A组大鼠肾脏AQP1、AQP2表达均较A-C组降低(P<0.05),M-V组、M-A组间差异无统计学意义(P<0.05)。结论骨髓MSC移植可增加血清白蛋白,降低尿蛋白、血清钠和肾实质细胞内AQP1、AQP2表达,对修复阿霉素CKD大鼠肾损伤有作用。在一定时间内肾动脉途径移植效果优于外周静脉途径,但两种移植途径的效果差异与AQP1、AQP2表达无明显关联。Objective To evaluate the therapeutic effect of bone marrow mesenchymal stem cell （MSC） infusion transplantation via renal artery and via caudal vein in treating chronic kidney disease （CKD） in rats, and to compare the expressions of aquaporinl （AQP1） and aquaporin2 （AQP2） between the two transplantation routes. Methods A total of 50 male SD rats were selected for this experiment. Two experimental rats were used to make preparation of bone marrow MSC. CKD model was established with infusion of adriamycin via caudal vein in 36 rats. The 36 CKD models were randomly divided into adriamycin-induced renal failure model control group （A-C group, n=12）, MSC transplantation through the right renal artery group （M-A group, n=12） and MSC transplantation through the caudal vein group （M-V group, n=12）. The remaining 12 male SD rats were used as the blank control group （N group）. One week after the last bone marrow MSC transplantation, the 24 h urine volume, 24 h urinary protein content, serum sodium content and serum albumin level were measured, and AQP1 and AQP2 expressions in the kidney tissue were determined by immunohistoehemistry. Results Compared with A-C group, the serum albumin level and 24h urine volume in both M-V group and M-A group were significantly increased （P〈0.05）, while 24h urinary protein content and serum sodium content were remarkably decreased （P〈0.05）. The 24h urinary protein content in the M-A group was obviously lower than that in the M-V group （P〈0.05）. The AQP1 and AQP2 expressions in the kidney tissue in both M-V group and M-A group were strikingly lower than those in the A-C group （P〈 0.05）, but no statistically significant differences in AQP1 and AQP2 expressions existed between the M-V group and the M-A group （P〉0.05）. Conclusion MSC transplantation can increase serum albumin, and lower urinary protein, serum sodium and the expressions of AQP1 and AQP2 in renal parenchymal cells, which has the effect on repairing renal injury of adr

关 键 词：骨髓间充质干细胞 移植途径 阿霉素肾病 AQP1 AQP2 

分 类 号：R692[医药卫生—泌尿科学]