HPLC-MS/MS法测定化妆品中α-羟基酸的含量  

Determination the content of α-Hydroxy Acids in cosmetics by HPLC-MS/MS

作　　者：陈海滨 李玲玲 王红梅 

机构地区：[1]厦门市食品药品质量检验研究院,厦门361012

出　　处：《药物分析杂志》2017年第11期2057-2061,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:采用高效液相色谱-串联质谱法建立化妆品中5种α-羟基酸,酒石酸、苹果酸、乳酸、柠檬酸、乙醇酸的含量测定方法。方法:采用ZORBAX SB-C_(18)(4.6 mm×75 mm,3.5μm)色谱柱,以0.1%甲酸水溶液-甲醇(98∶2)为流动相,流速0.4 m L·min^(-1),柱温25℃,电喷雾离子源(ESI),多反应离子检测(MRM)扫描方式进行检测。结果:酒石酸、苹果酸、乳酸、柠檬酸和乙醇酸质量浓度分别在10.07~100.7、2.005~20.05、48.72~487.2、1.009~10.09和101.8~1 018μg·m L^(-1)的范围内线性关系良好(r>0.997),3种浓度水平平均加样回收率分别在102.6%~115.0%、101.2%~105.2%、102.9%~108.3%、97.8%~98.8%和100.4%~113.3%范围内。4批样品中酒石酸、苹果酸均未检出,乙醇酸均有检出含量分别在3.31%~10.02%,1批样品检出乳酸含量为1.51%,1批样品检出柠檬酸含量为0.03%。结论:所建立的方法简便灵敏,重复性好,可用于化妆品中α-羟基酸的含量测定。Objective： To establish the method of the determination of five α -hydroxy acids, tartaric acid, mahc acid, lactic acid, citric acid and hydroxyacetic acid in cosmetics by HPLC-MS/MS. Methods： The sample was separated on the ZORBAX SB-Cls column（ 4.6 × 75 mm, 3.5 μm ）, with elution by 0.1% aqueous formic acidmethanol（ 98：2 ）. The flow rate was 0.4 mL · rain-1, and the column temperature was 25 ℃. The analytes were detected by tandem mass spectrometry with the electrospray ionization （ ESI ） source combined with multiple reaction monitoring （ MRM ） mode. Results： Linearity of tartaric acid, malic acid, lactic acid, citric acid and hydroxyacetic acid standards were established within the range of 10.07-100.7 μg· mL-1, 2.005-20.05 μg· mL-1, 48.72-487.2 μg· mL-1, 1.009-10.09 μg· mL-1 and 101.8-101 8 μg·mL-1 with the correlation coefficient above 0.997, respectively. The recoveries on three concentration levels were 102.6%-115.0%, 101.2%-105.2%, 102.9%-108.3%, 97.8%-98.8% and 100.4%-113.3%. Tartaric acid and malic acid were not detected in four samples. The contents of hydroxyacetic acid were 3.31%-10.02%. The content of lactic acid was 1.51% in one sample. The content of citric acid was 0.03% in one sample. Conclusion：The method is simple, sensitive and well repeatable. It can be used for the determination of α -hydroxy acids in cosmetics.

关 键 词：化妆品 Α-羟基酸 果酸 酒石酸 苹果酸 乳酸 柠檬酸 乙醇酸 高效液相色谱-串联质谱法 

分 类 号：R917[医药卫生—药物分析学]