黄连提取物的中药生物药剂学分类系统研究  

Study on biopharmaceutics classification system for Chinese materia medica of extract of Huanglian

作　　者：刘洋[1] 尹秀文[1] 王子禹 李雪莲[1] 潘孟[1] 李彦萍 董玲[1] 

机构地区：[1]北京中医药大学,北京100102

出　　处：《中国中药杂志》2017年第21期4127-4134,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81473362)

摘　　要：中药生物药剂学分类系统(CMMBCS)的优势之一,是将分类归属的研究层面从单成分扩展到中药多成分,进而从多成分扩展到中药整体研究层面。该研究以黄连提取物中的生物碱为主要研究对象,探究生物碱单一成分及黄连提取物中各生物碱成分的水溶解性及肠渗透性变化规律,并对黄连提取物整体做中药生物药剂学分类系统归属研究。其中采用经典摇瓶法和高效液相色谱法测定生物碱单一成分及提取物中各生物碱成分的溶解度变化及分类,采用在体单向肠灌流法进行生物碱吸收定量研究,同时采用自定义权重系数法对黄连提取物整体进行渗透系数计算。One of the advantages of biopharmaceutics classification system of Chinese materia medica （CMMBCS） is expanding the classification research level from single ingredient to multi-components of Chinese herb, and from multi-components research to holistic research of the Chinese materia medica. In present paper, the alkaloids of extract of huanglian were chosen as the main research object to explore their change rules in solubility and intestinal permeability of single-component and multi-components, and to determine the biopharmaceutical classification of extract of Huanglian from holistic level. The typical shake-flask method and HPLC were used to de- tect the solubility of single ingredient of alkaloids from extract of huanglian. The quantitative research of alkaloids in intestinal absorp- tion was measured in single-pass intestinal perfusion experiment while permeability coefficient of extract of huanglian was calculated by self-defined weight coefficient method.

关 键 词：黄连提取物 生物碱 溶解性 肠渗透性 中药生物药剂学分类系统 

分 类 号：R283.6[医药卫生—中药学]