刺齿凤尾蕨化学成分及其体外抗肿瘤活性研究  

Chemical constituents from Pteris dispar and their anti-tumor activity in vitro

作　　者：王佳娜[1] 张艳[1] 宋丽艳[1] 彭玉勃 李文静 

机构地区：[1]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007

出　　处：《中国中药杂志》2017年第21期4159-4164,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(31471923;31172009)

摘　　要：乙醇提取刺齿凤尾蕨药材,提取物依次经过有机溶剂萃取,聚酰胺柱色谱、葡聚糖凝胶柱色谱和制备型HPLC色谱分离得到10个对映-贝壳杉烷型二萜类化合物。采用核磁共振(NMR)和质谱(ESI-MS)技术鉴定获得的化合物分别为geopyxin B(1),geopyxin E(2),ent-11α-hydroxy-18-acetoxykaur-16-ene(3),ent-14β-hydroxy-18-acetoxykaur-16-ene(4),neolaxiflorin L(5),ent-3β,19-dihydroxy-kaur-16-ene(6),ent-3β-hydroxy-kaur-16-ene(7),7β,17-dihydroxy-16α-ent-kauran-19-oic acid 19-O-β-Dglucopyranoside ester(8),crotonkinin C(9)和crotonkinin C(10)。化合物1~10均为首次从刺齿凤尾蕨中获得。体外活性研究表明,化合物1和2对Bel-7402细胞具有抑制作用,IC50分别为7.50,10.60μmol·L-1;化合物1和2对Hep G2细胞具有抑制作用,IC50分别为6.68,11.80μmol·L-1。The dried whole plant of Pteris dispar were milled and extracted with 95% EtOH. The resulting dried extract was isolated by kinds of chromatographic column, including polyamide, Sephadex LH-20, preparative HPLC. As a result, ten diterpenes were iso- lated from the plant. By analyzing of ESI-MS and NMR spectroscopic data, the structures were established as geopyxin B（1 ） , geopyxin E（2）, ent-11α-hydroxy-18-acetoxykaur-16-ene（ 3 ） , ent-14β-hydroxy-18-acetoxykaur-16-ene（ 4 ） , neolaxiflorin L（5）, ent-3β,19-di- hydroxy-kaur-16-ene （ 6 ）, ent-3β-hydroxy-kaur-16-ene （ 7 ）, 7β, 17-dihydroxy-16ce-ent-kauran-19-oic acid 19-O-β-D-glucopyranoside ester（ 8）, crotonkinin C （9） and crotonkinin C （10）. Compounds 1-10 were obtained from P. dispar for the first time. Compounds 1 and 2 showed moderate activities against Bel-7402 with IC50 values of 7. 50 and 10. 60 μmol L-1 , and against HepG2 with IC50 values of 6. 68,11.80 μmol L-1, respectively.

关 键 词：刺齿凤尾蕨 对映-贝壳杉烷二萜 体外抗肿瘤活性 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]