饮水型砷暴露人群血液热休克蛋白70mRNA及热休克蛋白90mRNA的表达及意义  

Expression and significance of Heat shock proteins 70 and Heat shock proteins 90 mRNA of residents exposed to arsenic via drinking water

作　　者：刘建平[1] 崔娜 郭志伟 李艳红 夏雅娟 

机构地区：[1]包头市中心医院转化医学中心,包头014040 [2]内蒙古综合疾病预防控制中心砷中毒防治科,呼和浩特010031

出　　处：《中华地方病学杂志》2017年第11期787-791,共5页Chinese Journal of Endemiology

基　　金：国家自然科学基金（81360414、41230749）

摘　　要：目的检测饮水型砷暴露人群血液热休克蛋白（HSP）70mRNA及HSP90mRNA的表达情况，探讨HSP70mRNA及HSP90mRNA的表达与砷中毒之间的关系。方法2011年抽取内蒙古巴彦淖尔市两个乡（五原县胜丰乡、杭锦后旗沙海乡）饮水型砷中毒病区226人，按照饮水砷含量将其分为对照组（饮水砷〈10μg／L）、低剂量组（饮水砷10～〈100μg／L）、中剂量组（饮水砷100～〈200μg／L）、高剂量组（饮水砷≥200μg／L）。在进行流行病学调查的基础上，采用实时荧光定量PCR技术，检测调查人群血液HSP70mRNA及HSP90mRNA的表达水平，同时分析HSP70mRNA及HSP90mRNA的表达与水砷、尿砷、指甲砷之间的关系。结果①随砷暴露剂量的增高，HSP70mRNA的表达水平呈先升高后下降趋势。中剂量组[3．01×10^-3（1．95×10^-3）]、高剂量组血液HSP70mRNA[2．44×10^-3（1．72×10^-3）]与对照组『2．27×10^-3（1．09×10^-3）]比较，差异均有统计学意义（P均〈0．05）；中剂量组人群血液HSP70mRNA[3．01×10^-3（1．95×10^-3）]与低、高剂量组[2．38×10^-3（1．55×10^-3）、2．44×10^-3（1．72×10^-3）]比较，差异有统计学意义（P均〈0．05）。男性人群血液HSP70mRNA的表达略高于女性[2．71×10^-3（1．90×10^-3）比2．34×10^-3（1．35×10^-3），t=2．523，P〈0．05]。②随砷暴露剂量的增高，HSP90mRNA的表达水平呈先升高后下降趋势。中剂量组人群血液HSP90mRNA[1．29×10^-3（1．04×10^-3）]的表达与对照组人群[1．04×10^-2（0．83×10。）]比较差异有统计学意义（P〈0．05），高剂量组人群血液HSP90mRNA[1．19×10^-3（0．88×10^-3）]与低、中剂量组[1．23×10^-2（0．68×10-2）、1．29×10^-2（1．04×10^-2）]比较，差异均有统计学意义（P〈0．05）。HSP70mRNA相对表达量随尿砷暴露水平变化呈u型曲线回归关系（R2=0．031，P〈0．05），同时HSP90mRNA的表达与尿砷、指甲砷之间�Objective Through measuring and analyzing blood Heat shock proteins （HSP） 70 and HSP90 mRNA expression of different levels of arsenic exposure population, to explore the relationship between HSP70 and HSP90 mRNA expression and endemic arsenic poisoning. Methods The study subjects included a total of 226 residents exposed to arsenic via drinking water from two townships in the city of Bayannur （Shengfeng Township and Hangjinhouqishabai Township Wuyuan County） in Inner Mongolia Autonomous Region in 2011. The residents were divided into four groups according to drinking water arsenic concentration： control group （drinking water arsenic concentration 〈 10 μg/L）, low exposure group （drinking water arsenic concentration 10 - 〈 100 μg/L）, middle exposure group （drinking water arsenic concentration 100 - 〈 200 μg/L）, and high exposure group （drinking water arsenic concentration ≥200 μg/L）. Epidemiological investigation was carried out. Real-time PCR technology was used to detect the expression levels of blood HSP70 and HSP90 mRNA. And the relationships between theexpression of HSP70 and HSP90 mRNA and water arsenic and urinary arsenic levels were analyzed. Results （1）With the increase of arsenic concentration, the expression level of HSP70 mRNA increased first and then decreased. The HSP70 mRNA in the blood of high exposure group [2.44 × 10-3 0.72 × 10-3）] and middle exposure group [3.01 ×10-3 （1.95 × 10-3）] were significantly different from that in the control group [2.27 × 10-3 （1.09 × 10-3）, P 〈 0.05]. There was significant difference between the middle exposure group [3.01 × 10-3 0.95 × 10-3）] and the low and high exposure groups [2.38 × 10-3 （1.55 × 10-3）, 2.44 × 10-3 （1.72 × 10-3）, P 〈 0.05]. The expression of HSP70 mRNA in blood of male [2.71× 10-3 （1.90 × 10-3）] was slightly higher than that of female [2.34 × 10-3 （1.35 × 10-3）, t = 2.523, P 〈 0.05]. （2） With the increase of arsenic concentration, the expression level

关 键 词：砷中毒:HSP70 MRNA HSP90 MRNA 

分 类 号：R599.1[医药卫生—内科学]