砷暴露对小鼠皮肤氧化和抗氧化损伤及黑色素含量的影响  

Lipid peroxidation and anti-lipid peroxidation and the skin pigment in skin of C57BL/6 mice exposed to arsenic

作　　者：田凤[][1] 吕懿[1] 刘海芳[2] 王美[1] 赵倩[1] 杨川丽 陈晓萍 郑金平[1] 裴秋玲[1] 

机构地区：[1]山西医科大学公共卫生学院卫生毒理学教研室,太原030001 [2]山西医科大学公共卫生学院实验中心,太原030001

出　　处：《中华地方病学杂志》2017年第11期798-801,共4页Chinese Journal of Endemiology

基　　金：山西省科技基础条件平台建设项目（2011091005-0102）;山西省回国人员基金（200945）;山西省科技攻关项目（2006031087-11）

摘　　要：目的探讨亚砷酸钠对小鼠皮肤氧化和抗氧化能力及皮肤色素含量的影响。方法选取6周龄健康雄性C57BL／6小鼠40只，按体质量采用随机数字表法分为4组，每组10只。对照组自由饮用蒸馏水；实验组自由饮用含砷量分别为1、5、25mg／L的亚砷酸钠溶液（低、中、高剂量组），连续染砷6周，并于染砷结束前12d给予毛囊诱导处理。染砷结束后，取小鼠背部脱毛部位皮肤，亚硝酸盐法检测超氧化物歧化酶（SOD）活力，二硫双硝基苯甲酸法检测谷胱甘肽过氧化物酶（GSH-Px）活力，硫代巴比妥酸法检测丙二醛（MDA）含量。氢氧化钠（NaOH）溶解法测定皮肤黑色素含量。结果各组小鼠体质量和饮水量比较差异无统计学意义（F=0．119、0．363，P均〉0．05）。低、中、高剂量组皮肤SOD活力[（16．00±5．05）、（13．96±2．02）、（10．46±3．14）U／mgprot]和CSH-Px活力[（98．14±23．92）、（87．18±10．87）、（53．56±19．97）U／mgprot]均低于对照组[（20．36±4．82）、（119．34±33．14）U／mgprot，P均〈0．05]；中、高剂量组皮肤MDA水平[（9．09±2．04）、（11．48±2．21）nmol／mgprot]均高于对照组[（6．19±0．56）nmol／mgprot]和低剂量组[（6．52±1．67）nmol／mgprot，P均〈0．05]。各染砷组皮肤黑色素水平与对照组比较，差异无统计学意义（P均〉0．05）。结论砷暴露可致小鼠皮肤脂质过氧化损伤，且与皮肤抗氧化能力下降有关。未发现砷对皮肤黑色素含量有影响。Objective To study the effect of lipid peroxidation and anti-lipid peroxidation and the pigment in skin of C57BL/6 mice exposed to arsenic. Methods Forty male C57BL/6 mice were divided into four groups via the random number table method, ten mice in each group, and the mice were fed ad libitum drinking water containing arsenic at 0, 1, 5 and 25 mg/L concentrations for a period of 6 weeks, respectively. Twelve days before the end of the experiment, procedure of depilation was performed to induce anagen of hair cycle. On the 30th day of experiment, the activity of superoxide dismutase （SOD） was determined by nitrite method, the activity of glutathione peroxidase （GSH-Px） was determined by dithiobisobenzoic acid method, the content of malondialdehyde （MDA） in skin of C57BL/6 mice was determined by thiobarbituric acid method. Melanin was measured by NaOH dissolution method. Results No significant difference was found in body weight and water intaken between groups （F = 0.119, 0.363, P 〉 0.05）. The activity of SOD [（16.00 ± 5.05）, （13.96 ± 2.02）, （10.46 ±3.14） U/mg prot] in 1, 5 and 25 mg/L arsenic groups were all significantly lower than that in control group [（20.36 ± 4.82） U/mg prot, P 〈 0.05]. GSH-Px activity in 1, 5 and 25 mg/L arsenic groups [（98.14 ± 23.92）, （87.18 ±10.87）, （53.56 ± 19.97） U/rag prot[ were all significantly lower than that in control group [（119.34 ± 33.14） U/mg prot, P 〈 0.05]. While MDA levels in 5 and 25 mg/L arsenic groups [（9.09 ± 2.04）, （11.48 ± 2.21） nmol/mg prot] were significantly higher thanthat of control group [（6.19 ± 0.56） nmol/mg prot, P 〈 0.05] and that of 1 mg/L arsenic group [（6.52 ± 1.67） nmol/mg prot, P 〈 0.05）. No significant difference was found in melanin levels （P 〉 0.05）. Conclusions Lipid peroxidation and the decreasing of antioxidation may be one of the mechanisms of arsenic-induced skin damage. Arsenic-induced skin melanin content change is not found.

关 键 词：砷 脂质过氧化 抗氧化 黑色素 

分 类 号：R599.1[医药卫生—内科学]