DNA条形码技术在甘肃省啮齿动物鉴定中的应用  

Identification of rodent animals by DNA barcoding technology in Gansu Province

作　　者：郭丽民[1] 席进孝[1] 盖永志[1] 苗克军[1] 穆洮霞[1] 

机构地区：[1]甘肃省疾病预防控制中心鼠防科,兰州730020

出　　处：《中华地方病学杂志》2017年第11期802-805,共4页Chinese Journal of Endemiology

基　　金：国家卫生行业科研专项（201202012）;甘肃省卫生行业计划项目（gswst2015-37）

摘　　要：目的探讨DNA条形码技术在甘肃省啮齿动物鉴定中的应用，为建立甘肃省啮齿动物DNA条形码数据库提供资料。方法选取甘肃省鼠类肝脏或肌肉作为研究对象，运用DNA条形码技术检测动物标本线粒体细胞色素C氧化酶I亚基（coI）基因序列。结果共检测54份样本，种内遗传距离为0-2％，种间遗传距离为18％-30％；邻接法系统树能区分8个物种的类群。结论DNA条形码技术可用于啮齿动物鉴定，对于甘肃省自然疫源性疾病防治有重要意义。Objective To apply the DNA barcoding technology for identification of rodent animals and to establish a rodent animal DNA barcode database in Gansu Province. Methods A total of 54 rodent animals were detected. DNA barcoding technology was used to analyze the DNA mitochondrial cytochrome C oxidase subunit I （CO I） gene sequence in Gansu Province. Results The intra-specific genetic distance was 0 - 2% while the inter- specific distance ranged from 18% to 30%. Eight major clusters were apparently showed on a Neighbor joining tree. Conclusion DNA barcoding technology could overcome the shortcomings of the morphological identification, so it could be used to identify the rodent animals and has important implications for disease control and prevention in the natural focus of Gansu Province.

关 键 词：啮齿目 DNA条形码 线粒体细胞色素C氧化酶I亚基 鼠种鉴定 

分 类 号：R184.3[医药卫生—流行病学]