巨噬细胞移动抑制因子基因在胎盘组织中表达与妊娠期糖尿病的相关性  

Expression of macrophage migration inhibitory factor gene in placenta tissue and its correlation withgestational diabetes mellitus

作　　者：郑林 李超[1] 齐卫红[1] 乔炳龙[3] 赵涵[2] 周玉彬 卢彩霞[1] 

机构地区：[1]青岛大学医学院附属医院产科,266003 [2]青岛大学医学院附属医院病理科,266003 [3]青岛市立医院放射科

出　　处：《中华医学杂志》2017年第43期3388-3391,共4页National Medical Journal of China

基　　金：山东省医药卫生科技发展计划（2016WS0274）

摘　　要：目的探讨巨噬细胞移动抑制因子（MIF）基因表达与中国北方汉族人群妊娠期糖尿病（GDM）发病的相关性。方法采用免疫印迹方法分别检测同期来源于青岛大学附属医院产科及日照市东港区妇幼保健院产科的126例GDM孕妇（GDM组）和150名正常孕妇（健康对照组）胎盘组织中MIF的表达情况，同时检测两组研究对象血糖、胰岛素水平及其他临床理化指标，比较两组研究对象胎盘组织中MIF蛋白表达量以及MIF蛋白表达与血糖、胰岛素水平及胰岛素抵抗指数（HOMA—IR）和其他临床理化指标的相关性。结果GDM组胎盘组织MIF蛋白表达水平（平均灰度值）为（0．85±0．10）高于对照组（0．12±0．09），差异有统计学意义（P〈0．05）；并且GDM组胎盘组织MIF高表达与空腹血糖（FBG）及HOMA—IR的升高密切相关（均P〈0．001）。结论MIF在GDM孕妇胎盘组织中表达水平升高，并与GDM孕妇FBG及胰岛素抵抗相关，提示MIF在GDM发生发展中可能起重要作用。Objective To investigate the relationship between the expression of macrophage migration inhibitory factor （M1F） gene and the incidence of gestational diabetes mellitus （GDM） in Chinese Han population in northern China. Method This study was consisted of 126 GDM women and 150 cases of healthy subjects who came from the obstetrics department of Maternity Hospital of Qingdao University and Maternity and Child Care Hospital of Donggang District of Rizhao. The protein expression of MIF in placenta tissue was detected by Western blot method. The blood glucose, insulin levels and other clinical physicochemical index were tested. The differences of MIF, fasting blood-glucose （FBG）, fasting insulin （FIN） and the homeostasis model assessment of insulin resistance （HOMA-IR） were compared between the two groups of pregnant women. Result The level of MIF protein in GDM group （0. 85 ±0. 10） was higher than that in healthy pregnant group （ 0. 12 ± 0. 09 ）, with significant difference （ P 〈 O. 001 ）. Insulin resistance index in GDM group were higher than that in healthy pregnant group, with significant difference （P 〈 0. 001 ）. Conclusion The expression of MIF in placental tissues of GDM women was increased and correlated with insulin resistance in GDM patients, suggesting that MIF may play an important role in the occurrence and development of GDM.

关 键 词：巨噬细胞移动抑制因子 糖尿病 胎盘组织 基因表达 

分 类 号：R714.256[医药卫生—妇产科学]