2015年镇江地区832份腹泻样本诺如病毒感染状况及进化重组分析  

Analysis on Norovirus infection and evolutionary recombination in 832 diarrhea samples of Zhenjiang area in 2015

作　　者：郝世轩 徐虹[1] 宋寅生[1] 薛渊[1] 许金凤[1] 

机构地区：[1]镇江市疾病预防控制中心,江苏镇江212000

出　　处：《现代预防医学》2017年第22期4181-4185,共5页Modern Preventive Medicine

基　　金：镇江市卫生科技重点专项项目(SHW2015015);镇江市科技计划社会发展项目(SH2015083)

摘　　要：目的分析2015年镇江地区诺如病毒的感染状况及进化重组现象。方法收集2015年镇江地区哨点医院腹泻患者的粪便标本及突发疫情的病例标本,采用实时荧光定量PCR(q RT-PCR)方法进行诺如病毒GI/GII核酸检测,阳性样本采用逆转录PCR(RT-PCR)方法扩增诺如病毒基因的Rd Rp区和Capsid区,序列测定、基因分型,运用Mega软件和RDP软件进行序列进化和基因重组分析。结果 832份样本中诺如病毒阳性率为5.05%(42/832),GII组阳性率为4.81%(40/832),占95.24%,GII组在10~20岁的中青年中阳性率最高,达25.00%(14/56)。2015年诺如病毒的检出率以1月最高,11月、2月次之,以GII.17型所占比例最高为63.89%(23/36),新GII.17型变异株为最主要的流行株。主要的重组事件为诺如病毒GII.P12_GII.3型的镇江毒株ZJ111/2015/CHN和ZJ119/2015/CHN,是由加拿大的参考株U02030/Toronto/CA/GII.3和英国的参考株X86557/Lordsdale/UK/GII.4重组而成。结论 2015年镇江地区诺如病毒感染以GII组为主,中青年阳性率最高,新GII.17型变异株为最主要的流行基因型,主要的重组事件为GII.3与GII.4的重组。Objective To analyze the infection status and evolutionary recombination of Norovirus of Zhenjiang area in 2015. Methods The samples of fecal samples and sudden epidemic cases were collected from patients with diarrhea of sentinel hospital in Zhenjiang district in 2015. Real-time quantitative PCR （qRT-PCR） method was used to detect Norovirus RNA of GI/GII nucleic acid. The positive samples were amplified by reverse transcription-polymerase chain reaction （RT-PCR） for the RdRp region and Capsid region of Norovirus genes, sequencing, genotyping. Mega software and RDP software were used to analyze sequence evolution and gene recombination. Results The positive rate of Norovirus in 832 samples was 5.05% （42/ 832） and GII genogroup was 4.81% （40/832）, accounting for 95.24%. The positive rate of GII was the highest among the young people aged from 10 to 20, reaching 25.00% （14/56）. In 2015, the detection rate of Norovirus was the highest in January, followed by November and February, with the highest proportion of GII.17 was 63.89% （23/36）, and the new GII.17 variant was the most prevalent epidemic strain. The major recombinant events were Norovirus GII.P12 GII.3 genotype of Zhenjiang strains ZJ111/2015/CHN and ZJll9/2015/CHN, which were recombined from the Canadian reference strain U02030/Toronto/CMGII.3 and the British reference strain X86557/Lordsdale/UK/GII.4. Conclusion In 2015, Norovirus infection in Zhenjiang area was mainly in GII genogroup, and the positive rate was the highest in young and middle-aged, the new GII.17 variant was the most prevalent genotype, and the main recombination events were the recombination of GII.3 and GIL4.

关 键 词：诺如病毒 感染状况 进化重组 

分 类 号：R183.4[医药卫生—流行病学]