血清c-Myc与EB病毒抗体联合检测对鼻咽癌的诊断价值  

Diagnostic value of combined detection of serum c-Myc and EB virus antibodies in nasopharyngeal carcinoma

作　　者：曾江辉 兰会华[2] 朱旭 郑利平 杨兰 马升俊 

机构地区：[1]广西医科大学第三附属医院检验科,南宁530031 [2]广西壮族自治区人民医院检验科,南宁530021

出　　处：《广西医科大学学报》2017年第11期1564-1567,共4页Journal of Guangxi Medical University

基　　金：广西南宁市科学研究与技术开发计划项目(No.20133182);广西壮族自治区卫生厅自筹经费科研项目(No.Z2013401;No.Z2014458)

摘　　要：目的:探讨原癌基因c-Myc与EB病毒(EBV)抗体联合检测对鼻咽癌(NPC)的临床诊断价值。方法:采用酶联免疫吸附试验(ELISA)法对68例NPC患者(观察组)及20例健康体检者(对照组)血清中的c-Myc、EBV衣壳抗原IgA抗体(EBVCA-IgA)和EBV早期抗原IgA抗体(EBV-EA-IgA)水平进行检测,观察血清c-Myc、EBV抗体浓度与患者临床病理特征的关系,并采用受试者工作特征(ROC)曲线评估c-Myc、EBV-VCA-IgA和EBV-EA-IgA对NPC的诊断价值。结果:观察组患者血清c-Myc、EBV-VCA-IgA和EBV-EA-IgA浓度均明显高于对照组(均P<0.05)。晚期(Ⅲ~Ⅳ期)NPC患者血清中EBVVCA-IgA的浓度较早期(Ⅰ~Ⅱ期)高,T3~T4期较T1~T2期高(P<0.05);N1~N3期患者血清EBV-EA-IgA水平明显高于N0期(P<0.05)。单项检测c-Myc、EBV-VCA-IgA和EBV-EA-IgA的ROC曲线下面积(AUC)分别为0.671、0.844、0.697,三者联合检测的AUC为0.879。结论:c-Myc、EBV-VCA-IgA和EBV-EA-IgA联合检测对NPC的诊断具有较高的临床价值。Objective： To investigate the clinical value of combined detection of c-Myc and Epstein-Barr virus （EBV） antibodies in nasopharyngeal carcinoma（NPC）. Methods：The serum levels of c-Myc, EBV-VCA-IgA and EBV-EA-IgA were detected by ELISA in 68 patients with NPC and 20 healthy subjects. Results：The serum levels of c-Myc, EBV-VCA-IgA and EBV-EA-IgA in NPC patients were higher than those of controls （P〈 0. 05 ）. EBV-VCA-IgA was related to clinical stage and T stage （P〈0. 05）, EBV-EA-IgA was associated with N stage （P〈0. 05）. The areas under the ROC curve（AUC） of c-Myc, EBV-VCA-IgA and EBV-EA-IgA were 0. 671,0. 844 and 0. 697, respectively. The AUC of the combination detection of c-Myc, EBV-VCA-IgA and EBV-EA-Ig was 0. 879. Conclusion： Combined detection of c-Myc, EBV-VCA-IgA and EBV-EA-IgA was valuable in the diagnosis of NPC.

关 键 词：鼻咽癌 C-MYC EB病毒VCA-Ig A抗体 EB病毒EA-Ig A抗体 

分 类 号：R766.3[医药卫生—耳鼻咽喉科]