TLR2、TLR9及CD19、CD23在儿童传染性单核细胞增多症中的表达及意义  

Expressions and significance of TLR2,TLR9,CD19 and CD23 in children with infectious mononucleosis

作　　者：母发光 王强[1] 郑佳 房俊 

机构地区：[1]四川省医学科学院.四川省人民医院儿科,四川成都610072 [2]四川省医学科学院.四川省人民医院医学实验中心,四川成都610072

出　　处：《实用医院临床杂志》2017年第6期192-195,共4页Practical Journal of Clinical Medicine

摘　　要：目的了解传染性单核细胞增多症(infectious mononucleosis,IM)患儿不同时期外周血单个核细胞TLR2、TLR9mRNA和CD19、CD23的表达情况及其变化规律,探讨其在发病中的作用。方法回顾性分析2015年4月至2016年2月30例我院确诊为IM患儿的临床资料。新诊断IM的30例患儿为IM急性期组,其中26例(失访4例)经治疗临床症状体征消失,病程满1个月为IM恢复期组,同期常规体检健康的24例儿童为对照组。采用SYBRGreen I实时荧光定量PCR方法检测外周血单个核细胞TLR2 mRNA、TLR9 mRNA的表达。采用流式细胞术检测外周血单个核细胞中B细胞CD19+和永生B细胞CD19+CD23+的阳性表达率。结果 IM患儿急性期CD19^+及CD19^+CD23^+表达阳性率低于恢复期(P<0.05),且急性期和恢复期表达阳性率均低于对照组(P<0.05)。急性期TLR2 mRNA、TLR9 mRNA表达水平高于恢复期(P<0.05),且急性期和恢复期表达水平均高于对照组(P<0.05)。结论 TLR2、TLR9,CD19^+CD23^+、CD19^+在IM不同时期通过对免疫细胞的调节可能参与了IM发病。Objective To investigate the expressions and variations of TLR2, TLR9, CD19 and CD23 in peripheral blood mononuclear ceils of children with infectious mononucleosis （ IM ）, and to explore their role in the pathogenesis of the disease. Methods The clinical data of 30 newly diagnosed IM from April 2015 to February 2016 in our hospital were retrospectively analyzed. The 30 cases were taken as IM acute group. Among them,26 cases whose clinical signs and symptoms disappeared after treatment （4 cases lost follow-up） and course of disease lasted for about 1 month were taken as IM recovery group. Meanwhile ,24 children with normal physical examination were taken as control group. The expressions of TLR2 mRNA and TLR9 mRNA in peripheral blood mononuelear cells was detected by SYBR Green I real-time fluorescence quantitative PCR. Flow cytometry was used to detect the positive expression rates of B cells （CD19^＋） and immortalized B ceils （CD19^＋CD23^＋） in the peripheral blood mononuelear cells. Results The positive expression rate of C D19^＋ and C D19^＋ CD23^＋ in the acute period of children with IM was lower than that in the recovery period （P 〈 0. 05 ）, and the positive rate of expressions in acute period and recovery period were lower than that in control group （ P 〈 0. 05 ）. The expression levels of TLR2 mRNA and TLR9 mRNA in acute period was higher than that in recovery period （ P 〈 0. 05 ）, and the expression levels in a- cute period and recovery period were higher than that in control group （ P 〈 0.05）. Conclusion TLR2,TLR9, CD19 and CD23 may play an important role in the pathogenesis of IM in different stages.

关 键 词：传染性单核细胞增多症 Toll受体2 Toll受体9 CD19 CD23 儿童 

分 类 号：R512.7[医药卫生—内科学]