麻黄水煎液及拆分组分对肾阳虚水肿大鼠的影响  

Effect of Ephedrae Herba Decoction and Its Splitting Fractions in Rats with Kidney-Yang Deficiency and Edema

作　　者：李苗 曾梦楠[1] 张贝贝 樊慧[1] 吴广操 冯卫生[1,2] 匡海学 郑晓珂[1,2] 

机构地区：[1]河南中医药大学,郑州450046 [2]呼吸疾病诊疗与新药研发河南省协同创新中心,郑州450046 [3]黑龙江中医药大学,哈尔滨150040

出　　处：《中国实验方剂学杂志》2017年第23期91-96,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划(973计划)项目(2013CB531802)

摘　　要：目的:研究麻黄及各拆分组分对肾阳虚水肿模型大鼠的影响,探讨功效与药性之间的关系,并初步讨论其作用机制。方法:用氢化可的松联合盐酸多柔比星复制肾阳虚水肿大鼠模型,即造模第1,8天分别尾静脉注射盐酸多柔比星(4,3.5 mg·kg^(-1)),与此同时,连续15 d腹腔注射氢化可的松注射液(3.75 mg·kg^(-1)·d-1)进行造模。造模结束后,将造模成功大鼠分为模型组,桂附地黄丸组(1.4 g·kg^(-1)),麻黄水煎液低(1.17 g·kg^(-1)),中(2.34 g·kg^(-1)),高(4.68 g·kg^(-1))剂量组,麻黄生物碱(0.020 g·kg^(-1)),非生物碱(0.105 g·kg^(-1)),醇沉(0.132 g·kg^(-1)),挥发油(0.933 3×10-3m L·kg^(-1))组,同时设立正常组,各组大鼠分别给予相应的药物(10 m L·kg^(-1)),正常组和模型组给予同等体积生理盐水,连续灌胃给药28 d。给药结束后,大鼠代谢笼法检测24 h尿量;考马斯亮蓝G-250染料结合法(CBB法)检测各组大鼠24 h尿蛋白;酶联免疫吸附法(ELISA)测定检测血清环磷酸腺苷(c AMP),环磷酸鸟苷(c GMP),三碘甲状腺原氨酸(T3),甲状腺素(T4),雌二醇(E2),睾酮(T)含量;蛋白免疫印迹法(Western bolt)检测肾脏水通道蛋白1(AQP1),水通道蛋白2(AQP2)含量。结果:麻黄水煎液和生物碱拆分组分能够显著性增加大鼠24 h尿量,血清c AMP,T3,T4和E2的水平(P<0.05,P<0.01);麻黄水煎液和生物碱组分能够显著性降低大鼠24 h尿蛋白,c GMP,T的含量以及肾脏AQP1和AQP2的表达(P<0.05,P<0.01)。结论:麻黄具有显著的利水消肿功效,生物碱为最佳有效组分,且其利水消肿机制可能与降低肾脏AQP1和AQP2的表达有关。Objective： To study the effect of Ephedrae Herba and its splitting fractions on the rat models of kidney-Yang deficiency and edema, explore the relationship between efficacy and drug properties, and discuss its mechanism. Method： The rat models of kidney-Yang deficiency were established with hydrocortisone combined with doxorubicin hydrochloride. Doxorubicin hydrochloride （4, 3.5 mg·kg^-1） was injected into the tail vein at day 1 and day 8, at the same time, hydrocortisone （3.75 mg·kg^-1·d^-1） was injected intraperitoneally for 15 days. After modeling, the rats were divided into model group, Guifu Dihuang pill group （1.4 g·kg^-1） , Ephedrae Herba decoction low （1.17 g·kg^-1）, medium （2.34 g.kg-1） and high （4.68 g·kg^-1） dose groups, ephedra alkaloid （0. 020 g·kg^-1）, non-alkaloid （0. 105 g·kg^-1 ）, alcoholic acid （0. 132 g·kg^-1 ）, and volatile oil （0. 933 3 x measured by enzyme-linked immunosorbent assay （ELISA）. Western blot was used to detect the contents of aquaporin 1 （AQP1） and aquaporin 2 （AQP2）. Result ： Ephedrae Herba decoction and its splitting fractions could significantly increase the levels of 24 h urine output, cAMP, T3, T4 and E2 in serum （P 〈0.05, P 〈 0.01 ）; Ephedrae Herba decoction and alkaloid components could significantly reduce rat 24 h urinary protein, cGMP, T, AQP1 and AQP2 expression levels （P 〈 0.05, P 〈 0.01）. Conclusion： Ephedrae Herba has significant diuresis promoting and detumescence effects, and alkaloids are the best effective components. Its mechanism may be related to the reduction of renal AQP1 and AQP2 expression.

关 键 词：麻黄 生物碱组分 肾阳虚水肿 水通道蛋白 

分 类 号：R285.5[医药卫生—中药学]