天麻多糖对脑瘫幼鼠脑内神经递质的影响  

Effect of Gastrodiae Rhizoma Polysaccharide on Brain Neutral Transmitter in Immature Rats with Cerebral Palsy

作　　者：史华[1] 何琦[2] 娄元俊[1] 邵素菊 

机构地区：[1]河南省中医院,郑州450002 [2]河南中医学院,郑州450046

出　　处：《中国实验方剂学杂志》2017年第23期140-145,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家中医药管理局项目(2012228);河南省教育厅重点科研项目(15A360003)

摘　　要：目的:探讨天麻多糖(Gastrodiae Rhizoma polysaccharide,GRPS)对脑瘫幼鼠神经递质的影响及其机制。方法:58只SD幼鼠,除空白组外,48只幼鼠结扎左侧颈总动脉并缺氧1 h造脑瘫模型,成模大鼠随机分为模型组,施普善组(2.5 m L·kg-1),GRPS高、低剂量组(300,150 mg·kg-1)。给药21 d后,Y型迷宫及跳台实验检测大鼠记忆力。化学或酶联免疫吸附(ELISA)法检测大脑皮层及左侧海马一氧化氮(nitric oxide,NO),乙酰胆碱脂酶(acetylcholin esterase,ACHE),5-羟色胺(5-hydroxytryptamin,5-HT),去甲肾上腺素(noradrenaline,NE)和γ-氨基丁酸(γ-aminobutyric acid,GABA)水平。实时荧光定量PCR(Real-time PCR)及ELISA法检测大脑皮层及海马内皮型一氧化氮合酶(endothelial nitric oxide synthase,e NOS)表达及含量。苏木素-伊红(HE)染色观察右侧海马组织结构。结果:与空白组比较,模型大鼠行为学错误次数增加,跳台潜伏期减少,大脑皮层和海马中5-HT,NE和GABA下降,ACHE和Glu增加(P<0.05,P<0.01)。与模型组比,GRPS高和低剂量组大鼠行为学错误次数减少,跳台潜伏期增加(P<0.05,P<0.01)。GRPS高和低剂量组大鼠在大脑皮层中NO,NE,5-HT和e NOS增加,ACHE减少;GRPS高剂量组大鼠海马的NO,NE和e NOS增加,ACHE减少(P<0.05,P<0.01)。GRPS低高剂量组大鼠海马组织结构未见水肿,细胞排列整齐。结论:GRPS可以增强脑瘫幼鼠记忆力,其机制与增加大脑皮层及海马中NO,NE和5-HT含量,降低ACHE水平,增加e NOS表达,保护海马区组织有关。Objective： To analyze the effect of Gastrodiae Rhizoma polysaccharide （GRPS） on neutral transmitter in the immature rats with cerebral palsy. Method： A total of 58 SD immature rats were used in the experiment, and except in the blank control ones, common carotid arteries of all the left 48 rats were ligatured and combined with hypoxia treatment to establish cerebral palsy models in immature rats. The successfully modeled rats were randomly divided into model group, cerebrolysin group （2.5 mL·kg^-1） , GRPS low （150 mg·kg^-1） and high dose （300 mg·kg^-1） groups. After treatment for 21 days, Y maze and diving platform experiment were used to explore the memory ability of rats. The levels of nitric oxide （NO）, acetylcholine esterase （ACHE）, 5- hydroxytryptamin （5-HT）, noradrenaline （NE） and T-aminobutyric acid （GABA） in cerebral cortex and hippocampus were measured by using ELISA or chemical method. Real-time PCR and ELISA were used to detect the expression levels of endothelial nitric oxide synthase （eNOS） mRNA and protein in brain. HE staining was used to observe the hippocampus tissue structure. Result： As compared with normal blank group, the error number in behavior was significantly increased in model group rats; the step down latency was decreased; and also, the levels of 5-HT, NE and GABA in cerebral cortex and hippoeampus of model group rats were significantly decreased, while the levels of ACHE and Glu were increased significantly （P 〈 0.05, P 〈 0.01 ）. As compared with the model group, the levels of NO, NE, 5-HT, and eNOS in cerebral cortex and hippocampus of GRPS low and high dose group rats were increased, while the level of ACHE was decreased （P 〈 0.05, P 〈 0.01 ） ; the levels of NO, NE, and expression of eNOS in hippocampus of GRPS high dose group were increased, while the levels of ACHE were decreased （P 〈 0.05, P 〈 0.01 ）. The regular cell position was observed in hippocampus of GRPS low and high dose groups, without edema. Conc

关 键 词：天麻多糖 脑瘫 神经递质 内皮型一氧化氮合酶 大脑皮层 海马区 

分 类 号：R285.5[医药卫生—中药学]