小反刍兽疫病毒(PPRV)与蓝舌病病毒(BTV)双重荧光RT-PCR快速检测方法的建立  

Rapid Detection of the PPRV and BTV by Double Real-time RT-PCR

作　　者：李儒曙 苏惠龙 罗宝正 赵福振 薄清如 邵建宏 邓湘辉 黄铮 

机构地区：[1]珠海市动物卫生监督所,珠海519001 [2]珠海出入境检验检疫局检验检疫技术中心,珠海519015

出　　处：《病毒学报》2017年第6期886-891,共6页Chinese Journal of Virology

摘　　要：为了研制小反刍兽疫病毒(PPRV)与蓝舌病病毒(BTV)双重荧光RT-PCR快速检测试剂盒,根据GenBank公布的小反刍兽疫病毒、蓝舌病病毒的基因序列,设计两套特异性的引物和探针,建立基于Taqman探针的双重荧光RT-PCR快速检测小反刍兽疫病毒与蓝舌病病毒的方法。实验结果表明,该方法特异性好、灵敏度高,检测最低浓度为10拷贝/μL数量级阳性标准品。通过对临床样品的检测,证实本研究建立的检测方法具有较好的临床应用价值。In order to develop a rapid-detection kit for the Peste despetits ruminants virus （PPRV） and Bluetongue virus （BTV）, two sets of specific primers and probes were designed based on the nucleotide se- quences of the PPRV and BTV available in GenBank. Then we established a method of TaqManTM probe- based Double Real-time reverse transcription-polymerase chain reaction for rapid detection of the PPRV and BTV. This method had high specificity and sensitivity,and was applicable for the testing of positive standard sample with a minimum concentration of 10 eopies/μL. The detection of clinical samples con- firmed that this method could be applied clinically .

关 键 词：小反刍兽疫病毒(PPRV) 蓝舌病病毒(BTV) 双重荧光RT-PCR 

分 类 号：R373.9[医药卫生—病原生物学]