辅助型T细胞相关细胞因子在特发性血小板减少性紫癜模型小鼠中的变化和意义  

Changes and Significance of T Helper Cell Related Cytokines in ITP Mouse Model

作　　者：王秀娟[1] 赵芳[1] 刘颖[1] 王蕾[1] 王新有[1] 孙明玲[1] 张颖[1] 李玲[1] 郭新红[1] 

机构地区：[1]新疆医科大学第一附属医院血液病中心,新疆维吾尔自治区血液病研究所,乌鲁木齐830000

出　　处：《血栓与止血学》2017年第6期901-903,907,共4页Chinese Journal of Thrombosis and Hemostasis

摘　　要：目的探讨外周血辅助型T细胞分泌的细胞因子在特发性血小板减少性紫癜(ITP)模型小鼠的变化及意义;方法采用CBA方法分别检测14只正常小鼠,14只未治疗ITP模型小鼠及14只泼尼松治疗后ITP模型小鼠外周血血清中的IL-2、INF-γ(Th1)、IL-4、IL-10(Th2)、TGF-β1(Th3)和IL-17(Th17)细胞因子水平的变化;结果与正常组比较,模型组中的IFN-γ、IL-2(Th1)、IL-17(Th17)水平明显升高,IL-10、IL-4(Th2)、TGF-β1(Th3)水平明显降低,Th1/Th2比值增高(P均<0.05),泼尼松组与正常组比较,Th1、Th2、Th17和Th3型细胞因子水平及Th1/Th2比值无明显差异(P均>0.05);结论在ITP模型小鼠中存在细胞亚群平衡的偏移,表现为Th1、Th17型细胞相关细胞因子分泌亢进,Th2、Th3型细胞相关细胞因子分泌减低,提示辅助型T细胞相关细胞因子参与了ITP的发病机制。Objective To investigate the changes and significance of idiopathic thrombocytopenic purpura （ITP）model mice peripheral blood T helper cell secreted in cytokines;Methods Used the method of Ctyometric Beads Arrays to detect cytokines levels of changes of IL- 2 and INF-γ（ Th1）, IL- 4 and IL- 10 （Th2） ,TGF-β1 （Th3）,IL-17 （Th17）of 14 normal mice, 14 mice with untreated ITP and 14 treated with prednisone ITP mouse model ; Results Compared with the normal group, the leves of IFN-γ and IL-2 （ Th1 ）, IL- 17 （ Th17 ） ,the ratio of Th1/Th2 were elevated ; the level of IL- 10 and IL-4 （ Th2 ） ,TGF- β1 （ Th3 ） were desreased in model group（P 〈0.05 ）. Compared with the normal group,the levels of Th1,Th2,Th3 and Th17 type cell factors were not significantly different in prednisone group （P 〉 0.05 ） ;Conclusion The existence of cell subsets balance shift in ITP mouse model; Performance of Th1 and Th17 cytokines secretion hyperthyroidism, Th2 and Th3 cytokines secretion reduction, pointed to T helper cell eytokines involved in the pathogenesis of ITP.

关 键 词：特发性血小板减少性紫癜模型小鼠 辅助型T细胞 细胞因子 

分 类 号：R558[医药卫生—血液循环系统疾病]