川、黔、渝几所医院土生角蛋白降解真菌多样性分析  

Diversity of keratin-degrading fungi in soil samples from several hospitals in Guizhou,Sichuan and Chongqing,Southwest China

作　　者：张芝元[1] 郑欢[1] 韩燕峰[1] 陈万浩[1] 梁宗琦[1] 

机构地区：[1]贵州大学生命科学学院真菌资源研究所,贵州贵阳550025

出　　处：《菌物学报》2017年第11期1504-1513,共10页Mycosystema

基　　金：科技部基础工作专项(2013FY110400);国家自然科学基金(31460010);贵州省优秀青年人才培养对象专项(2013-05)~~

摘　　要：采用毛发钓饵技术,从几所医院采集的土样中分离获得角蛋白降解真菌774株。通过形态学特征、生物条码数据系统(Barcode of Life Data Systems)、UNITE数据库、CBS数据库和分子系统学分析的多途径方法,将这些菌株鉴定为31属58种。结合文献和ISHAM(International Society for Human and Animal Mycology)r DNA-ITS数据库的分析结果表明,链格孢属Alternaria、曲霉属Aspergillus、毛壳属Chaetomium、芽枝霉属Cladosporium、镰刀菌属Fusarium、地丝霉属Geomyces、地霉属Geotrichum、小孢属Microsporum、青霉属Penicillium、紫霉属Purpureocillium、篮状菌属Talaromyces、木霉属Trichoderma和毛癣菌属Trichophyton共13个属中的20种512株真菌对人类具有潜在感染风险。Seven hundred and seventy-four isolates of keratin-degrading fungi were isolated from soil samples collected in several hospitals by use of the hair-baiting technique. Fifty-eight species in 31 genera were identified through morphology, Barcode of Life Data Systems, UNITE Database, CBS Database and phylogenetic analysis. According to literatures and the International Society for Human and Animal Mycology rDNA-ITS Database, 512 isolates of 20 species in 13 genera （Alternaria, Aspergillus, Chaetomium, Cladosporium, Fusarium, Geomyces, Geotrichum, Microsporum, Penicillium, Purpureocillium, Talaromyces, Trichoderma and Trichophyton） were of potential pathogenicity to human.

关 键 词：医院 角蛋白降解真菌 潜在病原真菌 真菌多样性 

分 类 号：Q93[生物学—微生物学]