脂联素对软骨干细胞成软骨分化作用的影响  

Study on the effect of adiponectin on the differentiation of cartilage derived stem cells into chondrocytes

作　　者：左伟 程文俊[1] 焦竞[1] 黄玉成[1] 肖飞 王俊文[1] 

机构地区：[1]华中科技大学同济医学院附属普爱医院骨科,武汉430033

出　　处：《中华实验外科杂志》2017年第11期1824-1827,共4页Chinese Journal of Experimental Surgery

基　　金：湖北省自然科学基金（2017CFB255）;武汉市卫生与计划生育委员会基金（WX15C23）

摘　　要：目的从晚期骨关节炎患者关节软骨中分离出软骨干细胞，观察脂联素对软骨干细胞成软骨分化作用的影响。方法收集5例晚期骨关节炎患者行全膝关节置换术后剩余的软骨组织，培养分选出软骨干细胞，观察比较单纯成软骨诱导培养基组和加入了脂联素的成软骨诱导组成软骨诱导后组织Ⅰ型胶原、Ⅱ型胶原、蛋白聚糖（Agg）及性别决定区Y框蛋白-9（SOX-9）的免疫组织化学及相关基因的表达。 结果晚期骨关节炎患者关节软骨中分选出的软骨干细胞在适当条件下能向软骨细胞进行分化；当在成软骨诱导培养基中加入脂联素后，与未加脂联素的诱导组比较，细胞内Ⅱ型胶原和Agg的蛋白和基因表达减弱（Ⅱ型胶原：1.004±0.125比0.588±0.101，P＝0.001；Agg：1.002±0.050比0.584±0.138，P＝0.000）, SOX-9下调不明显（0.998±0.080比0.946±0.149，P＝0.513），但Ⅰ型胶原表达明显增强（2.290±0.349比1.010±0.059，P＝0.000）。结论脂联素对软骨干细胞的成软骨能力有一定抑制作用。Objective To observe the effect of adiponectin on the differentiation of cartilage derived stem cells into chondrocytes from the articular cartilage of patients with advanced osteoarthritis. Methods The remaining cartilage tissue was collected from 5 patients with advanced osteoarthritis under-went total knee arthroplasty, then cultured and selected chondroprogenitors, to observe and compare the ex- pression of type I , type II collagen, Aggrecan and sex determining region Y box - 9 （ SOX - 9 ） by im- munohistochemistry and the related genes expression between simple cartilage induced group and the group with adiponectin. Results The chondroprogenitors selected from articular cartilage of patients with ad- vanced osteoarthritis can differentiate into chondroeytes under appropriate conditions ; when in chondrogenie medium added adiponectin, compared with the group without adiponectin, the gene and protein expression of type II collagen and Aggrecan decreased （type II collagen： 1. 004 ±0. 125 vs. 0. 588 ±0. 101, P= 0. 001 ; Aggrecan： 1. 002 ± 0. 050 vs. 0. 584 ± 0. 138, P = 0. 000）, the SOX - 9 downregulation was not obvious （0. 998 ±0. 080 vs. 0.946±0. 149, P =0. 513）, but the expression of type I collagen increased significantly （ 2. 290±0. 349 vs. 1. 010 ± 0. 059, P = 0. 000 ）. Conclusion Adiponectin can inhibit chondrogenic potential of chondroprogenitors.

关 键 词：脂联素 软骨干细胞 成软骨分化 性别决定区Y框蛋白-9 

分 类 号：R684.3[医药卫生—骨科学]