水稻白叶枯病菌毒力基因tal17.5病理学功能的研究  

Functional characterization of tal17.5 gene from Xanthomonas oryzae pv.oryzae strain JXOV

作　　者：孙荣华 李融梅 何翔 宋从凤 

机构地区：[1]南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室,江苏南京210095

出　　处：《南京农业大学学报》2017年第6期1007-1013,共7页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(31471744);国家公益性行业(农业)科研专项(201303015)

摘　　要：[目的]类转录激活蛋白(transcription activator-like effectors,TALE)是稻黄单胞菌水稻致病变种(Xanthomonas oryzae pv.oryzae,Xoo)中的一类重要致病因子。挖掘新的毒力tal基因,对揭示水稻白叶枯病菌与寄主植物的互作关系具有重要意义。[方法]从水稻白叶枯病菌JXOV菌株中克隆了1个中间串联重复区含17.5个重复单元的tal基因,命名为tal17.5。将tal17.5转入Xoo菌株PXO99A中,在寄主水稻上研究了其致病力作用,将tal17.5转入ME2中研究了其诱导相关Os SWEET基因的表达情况;在非寄主烟草上研究了其对烟草防卫反应功能的影响。[结果]tal17.5导入PXO99A之后增强了PXO99A在所测试的22个水稻品种中的11个品种上的致病力,并减弱了PXO99A在烟草上的防卫反应,能够一定程度地诱导Os SWEET11和其他5个Os SWEET基因的表达。[结论]TAL17.5通过抑制植物的防卫反应发挥一定的毒力作用。[Objectives] Transcription activator-like effectors （ TALE ） are important factors in Xanthomonas oryzae pv. oryzae （ Xoo ）. This paper aims to explore new virulence tal genes and identify their pathological functions. [ Methods] We cloned a ta/gene named tallZ5 from Xoo JXOV strain,which encoded a typical TALE with 17.5 tandom repeat units, tallT.5 was transformed into ME2 and PXO99A respectively by electroporation. Leaf inoculation and quantitative real-time polymerase chain reaction in host plant and callose deposition induction in non-host tobacco were used in this study to compare the virulence of the transformant with wildtype. [ Results ] The results showed that the tall T.5 gene significantly increased the PXO99A virulence in 11 out of 22 tested rice varieties and suppressed the defense response in non-host Nicotiana benthamiana. Besides it enhanced six OsSWEET genes expression level in rice variety ＇ IRBB10＇. [ Conclusions] TALl7.5 may contribute as a virulence factor through suppressing the defense response of host plant flee.

关 键 词：稻黄单胞菌水稻致病变种 JXOV菌株 tal17.5基因 PX099^A菌株 毒力因子 

分 类 号：S432.1[农业科学—植物病理学]